ArticleMolecular biology reports2025
Investigation of the anti-inflammatory effects of photobiomodulation therapy on human gingival fibroblasts (HGF1-PI1).
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
introductionPeriodontal disease [1] is a chronic inflammatory condition caused by pathogens such as Porphyromonas gingivalis (Pg), which leads to destruction of periodontal structures. Photobiomodulation therapy (PBMT) holds promise in alleviating inflammation and oxidative stress, yet optimal parameters remain debatable. This study investigates the effects of 660 nm and 970 nm lasers on viability, inflammatory gene expression, and cytokine secretion in human gingival fibroblasts (HGF1-PI1) to assess PBMT's potential in PD treatment.
methodsHuman Gingival Fibroblasts (HGF1-PI1) were cultured in DMEM supplemented with 10% FBS. An inflammatory model was established using Porphyromonas gingivalis-derived lipopolysaccharide (LPS), with optimal concentrations chosen according to ROS/IL-6 production and cell viability using the MTT assay. Cells were exposed to 660 nm (50 mW) or 970 nm (200 mW) lasers at 6, 12, and 18 J/cm². Gene expression study of inflammation-associated genes, IFN-γ, IL-6, and TNF-α, was performed using RT-qPCR, while cytokine levels were evaluated by the ELISA technique.
resultsFindings from the MTT assay revealed no significant change in cell viability with laser treatment alone or combined with Pg.LPS, compared to the control group. Real-time PCR analysis for cytokine gene expression of IFN-γ, IL-6, and TNF-α indicating a decrease in gene expression in HGF cells treated with both Pg.LPS and laser. Inflammatory cytokine production, showed the highest levels in the Pg.LPS group, while the laser-treated and control groups demonstrated no significant difference.
conclusionPhotobiomodulation therapy using dual-wavelength diode lasers (660 and 970 nm) effectively attenuated Porphyromonas gingivalis LPS-induced inflammation in human gingival fibroblasts by downregulating key proinflammatory mediators, including IFN-γ, IL-6, and TNF-α. These findings suggest that PBMT may serve as a safe and supportive therapeutic approach for modulating inflammation in periodontal tissues.
Indexed as
Identifiers
41296079What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.