Evidence map›Paper›PMID 41294728›Full record

ArticleBiosensors2025

A Screening Assay for Bile Acid-Transforming Microorganisms Using Engineered Bacterial Biosensors.

Debora Dallera, Daniele Pastorelli, Massimo Bellato, Angelica Frusteri Chiacchiera, Francesca Usai, Maria Gabriella Cusella De Angelis, Paola Brun, Paolo Magni, Lorenzo Pasotti

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Debora DalleraDepartment of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.ORCID 0000-0001-8166-1879
Daniele PastorelliDepartment of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.
Massimo BellatoDepartment of Molecular Medicine, University of Padua, Via Gabelli 63, 35121 Padua, Italy.ORCID 0000-0001-7428-0656
Angelica Frusteri ChiacchieraDepartment of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.
Francesca UsaiDepartment of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.
Maria Gabriella Cusella De AngelisCentre for Health Technologies, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.ORCID 0000-0003-2642-3346
Paola BrunDepartment of Molecular Medicine, University of Padua, Via Gabelli 63, 35121 Padua, Italy.ORCID 0000-0002-3279-9000
Paolo MagniDepartment of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.ORCID 0000-0002-8931-4676
Lorenzo PasottiDepartment of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.ORCID 0000-0002-5431-3083

Funding

Fondazione Cariparo 2021-59576Ministero dell'università e della ricerca ECS00000036
6 · The paper itself

Abstract

Bile salt hydrolase (BSH) enables microbial-mediated deconjugation of bile acids (BAs) in the gastrointestinal tract. BSH enzymes initiate bile acid metabolism by catalyzing the first, essential deconjugation step. Due to the strict connection between dysregulations of the BA pool and human or animal diseases, identification and characterization of strains with BSH activity are relevant for both healthcare and agroindustry. However, current methods are expensive, poorly sensitive, or require complex procedures. Here, a BSH screening assay for cultivated microbes is proposed, based on a bacterial biosensor that reports the concentration of different BA types via fluorescence. Although the biosensor is broadly responsive to various bile acids, the assay was designed to guarantee specificity by testing individual primary BAs within controlled concentration ranges. The assay was evaluated on two recombinant

Indexed as

Bile Acids and SaltsBiosensing TechniquesLactobacillusAmidohydrolasesEscherichia coliHumansAmidohydrolasesBile Acids and Saltscholoylglycine hydrolasebacterial sensorbile saltscalibration curveEscherichia colifluorimetric assaylactic acid bacteriaspecificitysynthetic biologyTcpHTcpP

Identifiers

PMID41294728
PMCPMC12649884

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.