Evidence map›Paper›PMID 41294727›Full record

ArticleBiosensors2025

NanoArrayPAD-X: Nanoprobe Array and 3D-µPAD for the Simultaneous Detection of Respiratory Pathogens and Biomarkers at the Point of Care.

Andreu Vaquer, Francisco M Bouzada, Sofia Tejada, Antonio Clemente, Antonia Socias, Maria Aranda, Alberto Del Castillo, Joana Mena, Maria Montaner, Rocío Rodríguez and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Andreu VaquerMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.ORCID 0000-0002-7983-5881
Francisco M BouzadaMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.ORCID 0009-0008-5511-2719
Sofia TejadaMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.ORCID 0000-0002-2000-6587
Antonio ClementeGroup of Innovation in Immunopathology of Infections (GTERi), Health Research Institute of the Balearic Islands (IdISBa), 07120 Palma de Mallorca, Spain.ORCID 0000-0002-0412-1315
Antonia SociasMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.ORCID 0000-0002-7435-8115
Maria ArandaMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.
Alberto Del CastilloMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.
Joana MenaMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.
Maria MontanerMicrobiology Department, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.
Rocío RodríguezMicrobiology Department, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.
Estrella Rojo-MolineroCIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Antonio OliverCIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Marcio BorgesMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.ORCID 0000-0001-9929-3111
Roberto de la RicaMultidisciplinary Sepsis Group, Health Research Institute of Balearic Islands (IdISBa), Son Espases University Hospital, 07120 Palma de Mallorca, Spain.

Funding

Agencia Estatal de Investigación CNS2022-135324Health Research Institute of the Balearic Islands JR23/02Instituto de Salud Carlos III CP23/00151Instituto de Salud Carlos III FI21/00167
6 · The paper itself

Abstract

Microfluidic paper-based analytical devices (µPADs) are ideal for point-of-care diagnostics due to their low cost, compact size, and ease of use. However, current designs have limited multiplexing capabilities, making it difficult to simultaneously detect pathogens and biomarkers in the same sample. In this work, we introduce NanoArrayPAD-X, a novel µPAD design that combines wax-printed microfluidic networks with an array of nanoprobes for the simultaneous detection of multiple targets. This is achieved by distributing the sample through the microfluidic network containing X detection areas. There, targets are captured through physical interactions and recognized by specific antibody-coated nanoprobes released from the nanoprobe array. This generates X dots whose color depends on the concentration of the targets in the sample. A NanoArrayPAD-5 platform capable of detecting five targets was developed to aid in the diagnosis of ventilator-associated pneumonia (VAP). The sensor array could detect

Indexed as

Biosensing TechniquesPoint-of-Care SystemsBiomarkersEscherichia coliHumansKlebsiella pneumoniaeLab-On-A-Chip DevicesPaperPneumonia, Ventilator-AssociatedPseudomonas aeruginosaStaphylococcus aureusBiomarkersbacteriabiosensormultiplexedpaper-basedpneumoniaVAP

Identifiers

PMID41294727
PMCPMC12650703

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.