ArticleBiomimetics (Basel, Switzerland)2025
Development of Acellular Hepatic Scaffolds Through a Low-Cost Gravity-Assisted Perfusion Decellularization Method.
Article in Biomimetics (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Exploring the memory of the extracellular matrix using MASH-derived decellularized scaffolds.Journal of tissue engineeringArticle
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Authors and funding
10 authors.
Funding
Abstract
backgroundDeveloping reliable and cost-effective decellularization methods is critical for advancing tissue engineering and regenerative medicine, particularly in regions with limited access to specialized perfusion systems.
methodsThis study standardized a gravity-assisted perfusion protocol for rat liver decellularization, designed to operate without pumps or pressurized equipment. Adult Wistar rat livers were processed through a gravity-driven vascular flushing method and compared with a conventional immersion-based protocol. The resulting scaffolds were evaluated by macroscopic inspection, histological staining (Masson's trichrome), and residual DNA quantification.
resultsThe gravity-assisted perfusion method achieved more efficient cellular removal and superior preservation of extracellular matrix (ECM) integrity compared with immersion. Residual DNA levels were 3.7 ng/mg in perfused samples, 209.47 ng/mg in immersed samples, and 331.97 ng/mg in controls, confirming a statistically significant reduction (
conclusionsThis low-cost, reproducible, and technically simple system enables the generation of high-quality acellular hepatic scaffolds without mechanical pumps. Its accessibility and scalability make it suitable for laboratories with limited infrastructure and educational settings. Moreover, this gravity-assisted approach provides a foundation for future recellularization and preclinical studies aimed at developing bioengineered liver constructs for regenerative and transplant applications.
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Registered trials
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