ArticleMicrobiology spectrum2026
Developing a recombinase-aided amplification method combined with a lateral flow dipstick assay for rapid triplex detection of bovine coronavirus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus.
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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10 authors.
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Abstract
To establish a sensitive, simple rapid method for detecting bovine coronavirus (BCoV), infectious bovine rhinotracheitis virus (IBRV), and bovine viral diarrhea virus (BVDV). Recombinase-aided amplification (RAA) was used to amplify template DNA or cDNA, and a lateral flow dipstick (LFD) was used to interpret the results after amplification was completed. Seventy-three rectal and nasal bovine samples were tested to evaluate the performance of the RAA-LFD assay and were tested in parallel via polymerase chain reaction (PCR) and reverse transcription quantitative PCR (RT-qPCR) for comparison. The triplex RAA-LFD assay was completed within 20 min at 39°C. This method demonstrated reasonable specificity, with no cross-reactivity to bovine norovirus (BNoV), bovine rotavirus (BRV), bovine parainfluenza virus 3 (BPIV3), or bovine respiratory syncytial virus (BRSV). The detection thresholds for IBRV, BVDV, and BCoV are 3.20 × 10
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