Evidence map›Paper›PMID 41293823›Full record

ArticleCirculation research2026

Functional Genomics Link REST to Endothelial Plasticity and Atherosclerosis.

Maria Viskadourou, Sharjeel Chaudhry, Arianna Scalco, Paula Reventun, Pablo Toledano-Sanz, Roujin An, Nunzio Alcharani, Maria Delgado Marin, Abigail Fennell, Quanyi Zhao and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Maria Viskadourou *Division of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0009-0004-3883-8471
Sharjeel Chaudhry *Division of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0000-0001-8701-3979
Arianna ScalcoDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.
Paula ReventunDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.
Pablo Toledano-SanzDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.
Roujin AnDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0009-0006-6689-9671
Nunzio AlcharaniInstituto Ramón y Cajal de Investigación Sanitaria, Universidad Francisco de Vitoria, Madrid, Spain (N.A.).
Maria Delgado MarinFaculty of Medicine, IRYCIS, Department of Systems Biology, Universidad de Alcalá, Alcalá, Spain (M.D.M.).ORCID 0000-0002-3078-7321
Abigail FennellDepartment of Biomedical Engineering (R.A., A.F., A.B., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0009-0007-7621-7993
Quanyi ZhaoDivision of Cardiology, Department of Medicine, Stanford University, Palo Alto, CA (Q.Z., T.Q.).
William OsburnDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0000-0002-7472-3805
Andrew S McCallionDepartment of Genetic Medicine (A.S.M.), Johns Hopkins University, Baltimore, MD.
Thomas QuertermousDivision of Cardiology, Department of Medicine, Stanford University, Palo Alto, CA (Q.Z., T.Q.).ORCID 0000-0002-7645-9067
Alexis BattleDepartment of Biomedical Engineering (R.A., A.F., A.B., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0000-0002-5287-627X
Charles J LowensteinDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0000-0003-0485-7514
Marios ArvanitisDivision of Cardiology, Department of Medicine (M.V., S.C., A.S., P.R., P.T.-S., R.A., W.O., C.J.L., M.A.), Johns Hopkins University, Baltimore, MD.ORCID 0000-0001-8046-6123

Funding

From genotype to phenotype in a GWAS locus: the role of REST in atherosclerosisK08HL166690 · NHLBI · OHIO STATE UNIVERSITY · PI Marios Arvanitis · 2023 to 2026
$681k
NHLBI NIH HHS K08 HL166690
6 · The paper itself

Abstract

backgroundGenome-wide association studies have identified multiple novel loci that contribute to coronary artery disease pathogenesis, but the mechanisms of these associations remain largely unknown.

methodsIn this study, we used a multitrait colocalization approach to prioritize novel endothelial-specific loci for atherosclerosis. We combined computational methods with in vitro assays and mouse models to study one of those new loci targeting the gene REST (RE1-silencing transcription factor).

resultsA multitrait colocalization approach across expression quantitative trait loci in atherosclerosis-relevant cell types, followed by in vitro CRISPR interference, revealed that a conserved regulatory element in a chromosome 4 genetic locus increases the risk of coronary artery disease and decreases the expression of REST, a transcriptional repressor, in endothelial cells.

conclusionsIn summary, our data reveal the novel role of REST as a repressor in endothelial cells that functions to constitutively inhibit endothelial-to-mesenchymal transition and protect against atherosclerosis.

Indexed as

Aortic DiseasesAtherosclerosisCell PlasticityEndothelial CellsGenomicsRepressor ProteinsAnimalsAortaCells, CulturedHumansMaleMiceMice, Inbred C57BLMice, KnockoutPlaque, AtheroscleroticProprotein Convertase 9Pcsk9 protein, mouseProprotein Convertase 9RE1-Silencing Transcription FactorRepressor Proteinsaortaatherosclerosiscoronary artery diseaseendothelial cellsgenomics

Identifiers

PMID41293823
PMCPMC12908446

What OpenQuestion holds

Texttitle and abstract
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.