Evidence map›Paper›PMID 41293681›Full record

ArticleBiomedical optics express2025

High-throughput marker-free screening of cytoplasmic vacuoles in adherent cells via Fourier ptychographic microscopy.

Marika Valentino, Massimo d'Agostino, Fabrizio Licitra, Daniele Pirone, Fulvia Vitale, Anna Di Micco, Luigi Michele Pavone, Lisa Miccio, Vittorio Bianco, Pietro Ferraro

Abstract read
In one paragraph

Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Marika ValentinoNational Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), via Campi Flegrei 34, 80078, Pozzuoli, Italy.
Massimo d'AgostinoDMMBM, Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Via S. Pansini 5, 80131 Napoli, Italy.
Fabrizio LicitraNational Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), via Campi Flegrei 34, 80078, Pozzuoli, Italy.
Daniele PironeNational Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), via Campi Flegrei 34, 80078, Pozzuoli, Italy.
Fulvia VitaleDMMBM, Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Via S. Pansini 5, 80131 Napoli, Italy.
Anna Di MiccoDMMBM, Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Via S. Pansini 5, 80131 Napoli, Italy.
Luigi Michele PavoneDMMBM, Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Via S. Pansini 5, 80131 Napoli, Italy.
Lisa MiccioNational Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), via Campi Flegrei 34, 80078, Pozzuoli, Italy.
Vittorio BiancoNational Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), via Campi Flegrei 34, 80078, Pozzuoli, Italy.ORCID https://orcid.org/0000-0003-1956-4976
Pietro FerraroNational Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), via Campi Flegrei 34, 80078, Pozzuoli, Italy.ORCID https://orcid.org/0000-0002-0158-3856

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The formation of intracellular vacuoles in mammalian cells is associated with different dysfunctions, e.g., autophagy, lysosomal storage diseases, and cellular stress. We introduce a pipeline for high-throughput, label-free analysis of adherent cells and vacuoles using Fourier ptychographic microscopy (FPM). In fact, by tailoring the Cellpose model to the case of FPM phase-contrast imaging, we segment and characterize more than 3 × 10

Identifiers

PMID41293681
PMCPMC12643019

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.