Evidence map›Paper›PMID 41293454›Full record

ArticleTransboundary and emerging diseases2025

Development and Application of a TaqMan-Based One-Step Quadruplex Reverse Transcription Real-Time PCR (RT-qPCR) for Differential Detection of Four Porcine Diarrhea Viruses.

Wei Wang, Zhaokun Chen, Dandan Wang, Jizong Li, Baochao Fan, Xuehan Zhang, Min Sun, Yongxiang Zhao, Jinzhu Zhou, Hongqi Shang and 2 more

Abstract read
In one paragraph

Article in Transboundary and emerging diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Wei WangInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0002-0721-447X
Zhaokun ChenChina Animal Health and Epidemiology Center, Qingdao 266032, China.ORCID https://orcid.org/0009-0006-8483-6877
Dandan WangInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0009-0002-9158-8163
Jizong LiInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0001-5682-1834
Baochao FanInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0001-9780-5080
Xuehan ZhangInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0002-0647-2266
Min SunInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0002-9323-0758
Yongxiang ZhaoInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0002-7707-6981
Jinzhu ZhouInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0002-3786-5687
Hongqi ShangInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0003-2743-539X
Bin LiInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0000-0003-1318-7081
Junming ZhouInstitute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.ORCID https://orcid.org/0009-0006-1897-3718

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and porcine rotavirus group A (PoRVA) are recognized as major enteric viral pathogens responsible for porcine viral diarrhea. These viruses exhibit similar clinical manifestations, including vomiting, diarrhea, and dehydration, which complicate differential diagnosis. Therefore, there is an urgent need for a highly sensitive and specific diagnostic method to differentiate these pathogens. In this study, we developed a TaqMan-based one-step quadruplex reverse transcription real-time PCR (RT-qPCR) assay for the simultaneous and differential detection of these four porcine diarrhea viruses. The standard curves demonstrated correlation coefficients (

Indexed as

DeltacoronavirusDiarrheaPorcine epidemic diarrhea virusReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSwine DiseasesAnimalsRotavirusSensitivity and SpecificitySwineTransmissible gastroenteritis virusporcine deltacoronavirusporcine epidemic diarrhea virusporcine rotavirus group Aporcine viral diarrheaRT-qPCRtransmissible gastroenteritis virus

Identifiers

PMID41293454
PMCPMC12643720

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.