Evidence map›Paper›PMID 41292731›Full record

ArticlebioRxiv : the preprint server for biology2025

Decorin Promotes Nascent Proteoglycan Retention in Cartilage Matrix by Strengthening Collagen II-Aggrecan Integration.

Thomas Li, Gabriela Canziani, Yuchen Liu, Neil Patel, Biao Han, Mingyue Fan, Annie Porter, Bryan Kwok, Chao Wang, Qing Li and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Thomas LiSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Gabriela CanzianiDepartment of Biochemistry and Molecular Biology, College of Medicine, Drexel University, Philadelphia, PA 19102, United States.
Yuchen LiuSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Neil PatelSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Biao HanSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Mingyue FanSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Annie PorterDepartment of Mechanical Engineering, University of Delaware, Newark, DE 19716, United States.
Bryan KwokSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Chao WangSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
Qing LiSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.
David E BirkDepartment of Molecular Pharmacology and Physiology, Morsani School of Medicine, University of South Florida, Tampa, FL 33612, United States.
Renato V IozzoDepartment of Pathology and Genomic Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, United States.ORCID 0000-0002-5908-5112
Irwin M ChaikenDepartment of Biochemistry and Molecular Biology, College of Medicine, Drexel University, Philadelphia, PA 19102, United States.
X Lucas LuDepartment of Mechanical Engineering, University of Delaware, Newark, DE 19716, United States.
Robert L MauckMcKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States.
Lin HanSchool of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States.ORCID 0000-0003-4180-1288

Funding

Overall: Resource-based Center for Musculoskeletal Disorders Research (Overall Application)P30AR069619 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI LOUIS J SOSLOWSKY · 2016 to 2026
$9.1M
Biomechanics of Cartilage: Roles of Decorin in ECM Assembly and DegradationR01AR074490 · NIAMS · DREXEL UNIVERSITY · PI Lin Han · 2019 to 2026
$2.3M
Prevention of PTOA via regulation of the cytomechanics of chondrocytesR01AR074472 · NIAMS · UNIVERSITY OF DELAWARE · PI LU, X. LUCAS · 2020 to 2025
$1.5M
NIAMS NIH HHS P30 AR069619NIAMS NIH HHS R01 AR074472NIAMS NIH HHS R01 AR074490
6 · The paper itself

Abstract

Cartilage extracellular matrix (ECM), a hydrated collagen II-aggrecan composite, undergoes dynamic turnover during both normal homeostasis and disease-associated remodeling. This study elucidates a crucial role for decorin in promoting the retention and stability of nascent aggrecan within this matrix. By applying bio-orthogonal click-labeling, we demonstrate that loss of decorin accelerates the release of nascent aggrecan under both physiological and inflammatory conditions, without affecting its preferred localization to the pericellular matrix. Conversely, supplementation with exogenous decorin mitigates inflammation-induced loss of nascent aggrecan, supporting its potential as a therapeutic target. At the molecular level, decorin exhibits strong binding affinity for aggrecan, and enhances aggrecan-aggrecan and aggrecan-collagen II interactions, reinforcing its direct role in integrating cartilage matrix constituents. Also, by binding to collagen II, decorin stiffens the collagen II fibril network, thereby strengthening the confinement effect that limits the diffusive loss of entrapped aggrecan. Notably, decorin does not alter chondrocyte transcriptomic profiles

Indexed as

biomacromolecular assemblyclick-labelingDecorinextracellular matrixproteoglycans

Identifiers

PMID41292731
PMCPMC12642626

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.