Evidence map›Paper›PMID 41291933›Full record

ArticleEpigenetics & chromatin2025

ZAD mediates chromatin binding and insulator activity of Drosophila Pita and can be replaced with the human ZFP276 ZAD-like domain.

Yulia Vasileva, Olga Kyrchanova, Natalia Klimenko, Maria Necheukhina, Anna Fedotova, Oksana Maksimenko, Pavel Georgiev

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Article in Epigenetics & chromatin, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Yulia Vasileva *Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia.
Olga Kyrchanova *Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia.
Natalia Klimenko *Center for Genome Research, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia.
Maria NecheukhinaDepartment of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia.
Anna FedotovaDepartment of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia.
Oksana MaksimenkoCenter for Genome Research, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia. maksog@mail.ru.
Pavel GeorgievDepartment of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, 119334, Russia. georgiev_p@mail.ru.

Funding

Russian Science Foundation 19-74-30026-P
6 · The paper itself

Abstract

backgroundThe zinc finger-associated domain (ZAD), found in numerous Drosophila architectural proteins, such as Pita, enables homodimerization. Despite its prevalence in insects, only one human protein, ZFP276, possesses a ZAD-like domain. To date, the role of Pita has been studied in the formation of the boundaries of regulatory domains in the Bithorax complex, and the functional significance of its ZAD remains unclear.

resultsUsing CRISPR/Cas9-mediated pita replacement with an attP site, we generated flies expressing modified Pita variants. Null pita mutants die in the late stages of embryogenesis. Flies expressing Pita lacking ZAD, Pita

conclusionsZAD is critical for the insulator activity of Pita and its ability to efficiently bind to specific genomic regions. The human ZFP276 ZAD-like domain may function similarly to the ZAD of Pita, raising the question of why ZADs spread in insects but not in mammals.

Indexed as

ChromatinDNA-Binding ProteinsDrosophila ProteinsInsulator ElementsTranscription FactorsAnimalsDrosophila melanogasterHumansMicrotubule-Associated ProteinsNuclear ProteinsProtein BindingProtein DomainsChromatinCP190 protein, DrosophilaDNA-Binding ProteinsDrosophila ProteinsMicrotubule-Associated ProteinsNuclear ProteinsTranscription FactorsAbd-BArchitectural C2H2 proteinsBithoraxChromatin insulatorCP190Domain boundaryHousekeeping geneZinc finger-associated domainZinc-finger protein

Identifiers

PMID41291933
PMCPMC12649048

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