ArticleReproductive biology and endocrinology : RB&E2025
Changes in the interactions between mural granulosa cells and cumulus cells during ovulation after the LH surge based on transcriptome analyses.
Article in Reproductive biology and endocrinology : RB&E, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Changes in the interactions between mural granulosa cells and cumulus cells during ovulation after the LH surge based on transcriptome analyses.Reproductive biology and endocrinology : RB&E · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
backgroundThe luteinizing hormone (LH) surge during ovulation induces dynamic changes in cellular functions of mural granulosa cells (MGCs) and cumulus cells (CCs). However, the mechanisms by which the two cell types interact with each other and regulate their cellular functions remain unclear. In this study, we investigated transcriptomic changes in both cell types in order to reveal the cell-cell interactions between MGCs and CCs during the ovulatory process.
methodsMGCs and CCs were collected from mice treated with equine chorionic gonadotropin (eCG), at 0 h (before), and 4 and 12 h after human chorionic gonadotropin (hCG) injection. Transcriptomes of both cell types were obtained by RNA sequencing. The changes in cellular functions and cell-cell interactions were investigated by gene ontology (GO) analysis and interactome analysis, respectively. To validate the predicted interactions, MGCs and COCs collected 48 h after eCG injection were cocultured for 12 h, after which gene expression and COC expansion were assessed.
resultsFrom 0 to 4 h after hCG injection, many cellular functions, including steroidogenesis, angiogenesis, follicle rupture, inflammatory response and cumulus-oocyte complex (COC) expansion were activated by cell-cell interactions, most of which were bidirectional interactions between MGCs and CCs. From 4 h to 12 h, cell-cell interactions regulating angiogenesis, follicle rupture, and inflammatory response remained activated, while those regulating steroidogenesis and COC expansion were attenuated. The coculture model revealed that COC expansion was induced in the presence of MGCs. Furthermore, the expressions of genes related to steroidogenesis, angiogenesis, and COC expansion in CCs increased in the presence of MGCs while their expressions in MGCs increased in the presence of CCs.
conclusionsInteractions between MGCs and CCs regulate the dynamic and time-dependent changes in their cellular functions during the ovulatory process, highlighting their essential regulatory roles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.