ArticleBiotechnology for biofuels and bioproducts2025
Optimising environmental factors for maximal lactate productivity in Synechocystis sp. PCC 6803 through a design of experiments approach.
Article in Biotechnology for biofuels and bioproducts, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Genetic and metabolic engineering of Synechocystis sp. PCC 6803 has given rise to strains that produce a variety of bio-chemicals. Despite these successes, improvements in productivity metrics are required to achieve economically viable bio-production of carbon dioxide-derived compounds. Previously, environmental factors have been leveraged to increase product yields. Here, we optimise multiple environmental factors simultaneously using Design of Experiments (DOE) principles and find conditions that maximise L-lactate production. Light intensity, glycerol concentration, and light-dark cycle were found to be significant factors. Optimising these conditions resulted in a 6.3- and 7.4-fold increase in titre and yield. The results detailed here could have implications for metabolic engineering of, and bioprocesses using, Synechocystis sp. PCC 6803. In the future, DOE-mediated optimisation of environmental conditions could effectively maximise product titres from different production strains, or the enhanced conditions described here could be directly implemented in other metabolic engineering projects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.