Evidence map›Paper›PMID 41291766›Full record

ArticleCell communication and signaling : CCS2025

The polybasic puzzle: how N-terminal charges modulate Gαi

Paweł Mystek, Atte Martikainen, Ewa Błasiak, Waldemar Kulig, Agnieszka Polit

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Paweł Mystek *Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland.
Atte Martikainen *Department of Physics, University of Helsinki, P.O. Box 64, Helsinki, FI-00014, Finland.
Ewa BłasiakDepartment of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland.
Waldemar KuligDepartment of Physics, University of Helsinki, P.O. Box 64, Helsinki, FI-00014, Finland. waldemar.kulig@helsinki.fi.
Agnieszka PolitDepartment of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland. a.polit@uj.edu.pl.

Funding

Academy of Finland 331349, 336234, 346135Polish National Science Centre 2016/23/B/NZ1/00530
6 · The paper itself

Abstract

G protein-coupled receptors (GPCRs) mediate essential cellular responses through interactions with heterotrimeric G proteins, particularly the Gαi subfamily. Despite high sequence similarity among Gαi subunits, subtle differences in their N-terminal polybasic regions influence membrane association, heterotrimer stability, and downstream signaling. Here, we investigate the functional significance of two arginine residues (R21 and R32) within the N-terminal helix of Gαi

Indexed as

Cell MembraneGTP-Binding Protein alpha Subunits, Gi-GoSignal TransductionCyclic AMPHEK293 CellsHumansMolecular Dynamics SimulationStatic ElectricityCyclic AMPGTP-Binding Protein alpha Subunits, Gi-GoDopamine D2 receptorFluorescence microscopyGPCRGαi subunitHeterotrimeric g proteinsLipid–protein interactionsMolecular dynamics simulations

Identifiers

PMID41291766
PMCPMC12751887

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.