Evidence map›Paper›PMID 41291543›Full record

ArticleBMC cancer2025

Bone marrow aspirate Cit-H3 was identified as a novel biomarker of minimal residual disease in neuroblastoma.

Mengxia Zhang, Xiang Wang, Lin Liu, Yuge Ni, Yuanyuan Zhang, Xiaoyan Hu, Chen Huang, Baocheng Gong, Tiantian She, Chong Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mengxia ZhangDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Xiang WangDepartment of Medical Technology, Tianjin Medical University, Tianjin, China.
Lin LiuDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Yuge NiDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Yuanyuan ZhangDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Xiaoyan HuDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Chen HuangDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Baocheng GongDepartment of Pediatric Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
Tiantian SheDepartment of Medical Technology, Tianjin Medical University, Tianjin, China. tiantianshe@tmu.edu.cn.
Chong ChenDepartment of Clinical Laboratory, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China. chongchen@tmu.edu.cn.

Funding

National Natural Science Foundation of China No. 82202595Tianjin Health Research project No. TJWJ2021MS010Tianjin Municipal Education Commission No. 2021KJ262
6 · The paper itself

Abstract

backgroundNeuroblastoma (NB) is the most prevalent extracranial solid tumor in children. Neutrophils release neutrophil extracellular traps (NETs) following intense or prolonged activation. Neutrophil elastase (ELA2) and citrullinated histone H3 (Cit-H3) are specific markers of NETs. Additionally, neuron-specific enolase (NSE) serves as a biomarker and can be used to monitor the condition of NB progression, assess treatment response, and predict recurrence.

methodsThis study employed a retrospective study design, and all samples were collected prior to bone marrow aspiration for diagnosis. A total of 39 serum samples from patients diagnosed with NB by postoperative pathological bone marrow aspiration were included as the study subjects, and neutrophils, ELA2, and Cit-H3 were detected in the study subjects using ELISA. The blood routine data of 99 patients diagnosed with NB were collected, and these data were from patients different from those of the aforementioned 39 serum samples. And patients were divided into two groups, namely NB patients with positive bone marrow metastasis and those without metastasis, based on the result of bone marrow GD2 immunohistochemical staining. All data were statistically analyzed using IBM SPSS Statistics 26.0 and GraphPad Prism 9.0 software.

resultsA positive correlation was identified between NB bone marrow minimal residual disease (MRD) positivity and haematological indices, including interleukin-2 (IL-2) (p = 0.021), IL-6 (p = 0.030), NSE (p < 0.001), and Cit-H3 (p < 0.001). And MRD was positively associated with blood neutrophils in bone marrow (p < 0.05).

conclusionsThis study demonstrates a significant correlation between NETs and MRDs, and found a positive correlation between bone marrow MRD

Indexed as

Biomarkers, TumorBone MarrowHistonesNeoplasm, ResidualNeuroblastomaAdolescentChildChild, PreschoolExtracellular TrapsFemaleHumansInfantLeukocyte ElastaseMaleNeutrophilsPhosphopyruvate HydrataseBiomarkers, TumorELANE protein, humanHistonesLeukocyte ElastasePhosphopyruvate HydrataseCit-H3Minimal residual diseaseNeuroblastomaNeutrophil extracellular trapsNSE

Identifiers

PMID41291543
PMCPMC12751149

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.