Evidence map›Paper›PMID 41291443›Full record

ArticleBMC gastroenterology2025

Analysis of perioperative chemotherapy-mediated genomic changes in gastric cancer.

Ko Ikegame, Hayato Omori, Masao Hada, Hideki Watanabe, Atsushi Takano, Ayako Kimura, Masayuki Inoue, Kazushige Furuya, Michiya Yasutome, Yuji Iimuro and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC gastroenterology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Host andGut microbes · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Ko IkegameDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan. ko.ikegame@outlook.jp.
Hayato OmoriDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Masao HadaDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Hideki WatanabeDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Atsushi TakanoDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Ayako KimuraDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Masayuki InoueDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Kazushige FuruyaDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Michiya YasutomeDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Yuji IimuroDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Hiroshi NakagomiDepartment of Surgery, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Yamanashi, 400-8506, Japan.
Kenji AmemiyaGenome Analysis Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, 400-8506, Japan.
Yosuke HirotsuGenome Analysis Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, 400-8506, Japan.
Hitoshi MochizukiGenome Analysis Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, 400-8506, Japan.
Masao OmataThe University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSurgery remains the mainstay of treatment for advanced gastric cancer, but in recent years perioperative chemotherapy has been administered in an attempt to improve treatment results. The Cancer Genome Atlas (TCGA) has illuminated the molecular landscape of gastric cancer. However, genomic changes before and after perioperative chemotherapy and the associated effects on treatment resistance remain unclear. This study aimed to clarify genomic change in gastric cancers treated with perioperative chemotherapy.

methodsOf the 532 patients who underwent gastrectomy for gastric cancer between January 2015 and December 2020, this study included eight patients who received neoadjuvant chemotherapy (NAC). We collected biopsy samples before NAC and surgical samples after NAC. Recurrent tumor biopsy samples after adjuvant chemotherapy were also collected in two cases. DNA and RNA were extracted from these samples and analyzed by next-generation sequencing.

resultsMost of the oncogenic mutations found before NAC (TP53, CDH1, KRAS, PIK3CA, RNF43, and SMAD4) were also found in the post-NAC surgical sample. Several gene mutations with low allele frequency were lost or gained. In the recurrent biopsy samples, gene mutations shared before NAC and after NAC were also detected. In addition, some gene mutations were acquired as new mutations following surgery. Gene expression analysis showed genes related to the MAPK signaling pathway were overexpressed in the group without recurrence.

conclusionsMost of the oncogenic mutations were maintained throughout perioperative chemotherapy and remained in recurrent tumors. The development of drugs that affect oncogenic mutations during perioperative chemotherapy is required.

Indexed as

MutationStomach NeoplasmsAgedAntigens, CDCadherinsChemotherapy, AdjuvantClass I Phosphatidylinositol 3-KinasesFemaleGastrectomyHigh-Throughput Nucleotide SequencingHumansMaleMiddle AgedNeoadjuvant TherapyProto-Oncogene Proteins p21(ras)Smad4 ProteinAntigens, CDCadherinsCDH1 protein, humanClass I Phosphatidylinositol 3-KinasesKRAS protein, humanPIK3CA protein, humanProto-Oncogene Proteins p21(ras)Smad4 ProteinSMAD4 protein, humanTP53 protein, humanTumor Suppressor Protein p53Ubiquitin-Protein LigasesGastric cancerGenomic analysisPerioperative chemotherapy

Identifiers

PMID41291443
PMCPMC12648925

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.