Evidence map›Paper›PMID 41291345›Full record

ArticlePlant cell reports2025

Improving soybean fatty acid profiles by CRISPR/Cas12a-mediated gene editing of GmFAD2 and GmFAD3.

Zeru Wang, Yaxuan Zhang, Ning Xu, Longxiaoran Liu, Menghua Zhang, Shuangyan Huang, Chenyu Su, Tengfei Liu, Kaixuan Duan

Abstract read
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In one paragraph

Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zeru Wang *State Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Yaxuan Zhang *State Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Ning Xu *State Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Longxiaoran LiuState Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Menghua ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Shuangyan HuangState Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Chenyu SuState Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Tengfei LiuState Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China.
Kaixuan DuanState Key Laboratory of Agricultural and Forestry Biosecurity, Nanjing Agricultural University, Nanjing, China. duank@njau.edu.cn.ORCID http://orcid.org/0000-0003-4803-240X

Funding

National Natural Science Foundation of China 32172499Shuangchuang Program of Jiangsu Province JSSCTD202342
6 · The paper itself

Abstract

key messageUsing CRISPR/Cas12a, we engineered novel soybean germplasms by knocking out GmFAD2 (GmFAD2-1A, GmFAD2-1B) and GmFAD3 (GmFAD3A, GmFAD3B) genes, yielding elevated oleic or linoleic acid content. Soybean oil contains high levels of polyunsaturated fatty acids (PUFAs), which are known to reduce cholesterol levels and help prevent hypertension, thereby contributing significantly to human health. However, the chemical instability of PUFAs makes them susceptible to oxidation, a process that generates harmful trans-fatty acids. To address this issue, precise modulation of fatty acid composition in soybeans becomes critically important for health applications. In this study, we employed CRISPR/Cas12a gene editing technology to selectively knock out the GmFAD2 (GmFAD2-1A, GmFAD2-1B) and GmFAD3 (GmFAD3A, GmFAD3B) genes in soybean. This approach successfully created novel soybean germplasms with distinct fatty acid profiles: one with elevated oleic acid content and another with increased linoleic acid levels. These engineered variants provide valuable options for utilizing soybean oil with optimized fatty acid compositions tailored for specific health and nutritional purposes.

Indexed as

CRISPR-Cas SystemsFatty AcidsGene EditingGlycine maxPlant ProteinsFatty Acid DesaturasesLinoleic AcidOleic AcidPlants, Genetically ModifiedSoybean OilFatty Acid DesaturasesFatty AcidsLinoleic AcidOleic AcidPlant ProteinsSoybean OilCRISPR/Cas12aFatty acidGmFAD2GmFAD3Soybean

Identifiers

PMID41291345

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.