ArticleCommunications biology2025
Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
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Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exosome Biogenesis: Meta-Analysis of Intraluminal Vesicle Size Across Species.International journal of molecular sciences · 2026Pooled it
- Electrons and X-rays for diffraction and imaging.Acta crystallographica. Section D, Structural biology · 2026Review
- Radiation-dose effects in correlative X-ray/cryo-electron microscopy of frozen-hydrated biological samples.Acta crystallographica. Section D, Structural biology · 2026Article
- Demonstrating soft X-ray tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy.Scientific reports · 2025Article
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Authors and funding
8 authors.
Funding
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Abstract
Cryo-imaging in cellular biology provides the means to visualize the cellular interior at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent microscopy (cryo-VLFM) providing the specificity by tagging macromolecules or structures and cryo-electron tomography (cryo-ET) for the structural details at molecular level. The large resolution gap between these techniques, however, is limiting this correlative workflow as cryo-ET targets are often smaller than the resolution limit of cryo-VLFM. Here we introduce cryo-soft X-ray tomography (cryo-SXT) as an intermediate step that can compensate for the partial view caused by the lost cellular material due to FIB-milling and limited resolution of cryo-VLFM by providing invaluable cellular context information in 3D to the cryo-ET dataset within an integrated workflow. This work shows that X-ray and electron imaging are not mutually exclusive, creating opportunities for further correlative imaging strategies.
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Registered trials
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