Evidence map›Paper›PMID 41290969›Full record

ArticleScientific reports2025

Bioefficacy of Trichoderma citrinoviride against some plant pathogenic fungi.

Nesrain Abdulkareem Al-Mekhlafi, Amira Ali Al-Harethi, Nada Yahya Alzanam

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Mechanisms of action and biocontrol potential ofFrontiers in fungal biology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nesrain Abdulkareem Al-MekhlafiDepartment of Biological Sciences, Faculty of Science, Sana'a University, Sana'a, Yemen. n.almikhlafi@su.edu.ye.
Amira Ali Al-HarethiDepartment of Biological Sciences, Faculty of Science, Sana'a University, Sana'a, Yemen.
Nada Yahya AlzanamDepartment of Biological Sciences, Faculty of Science, Sana'a University, Sana'a, Yemen.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trichoderma spp. are widely recognized as effective biocontrol agents used to control plant pests and reduce reliance on chemical pesticides. This study aimed to evaluate the in vitro antifungal activity of Trichoderma citrinoviride (AUMIC 13831) against five common plant pathogens: Alternaria sp., Aspergillus sp., Colletotrichum sp., Fusarium sp., and Rhizoctonia sp. Antagonistic potential was assessed using dual culture, volatile metabolite, non-volatile metabolite, and slide culture assays. T. citrinoviride exhibited significant antagonism across all methods tested. In the dual culture method, T. citrinoviride effectively inhibited the radial growth of all tested pathogens, showing inhibition percentages ranging from 50.88% ± 0.73 (against Rhizoctonia sp.) up to a maximum of 61.29% ± 1.86 (against Colletotrichum sp.). The non-volatile metabolites extracted from the culture filtrate proved to be the most potent fungistatic mechanism, demonstrating the highest overall efficiency, particularly against Fusarium sp. (80.95% ± 0.92) and Colletotrichum sp. (69.62% ± 0.72). The volatile metabolite assay exhibited moderate fungistatic effects, achieving a maximum growth inhibition of 36.36% ± 0.43(against Alternaria sp.). Furthermore, the mycoparasitic interaction between T. citrinoviride and the pathogenic fungi was confirmed through detailed microscopic observations. T. citrinoviride shows promising in vitro antifungal activity across multiple antagonistic mechanisms. Further studies in planta, toxicological/environmental assessments, and detailed metabolite identification using LC-MS are required to confirm its applicability as a commercial biocontrol agent. Further studies under greenhouse or field conditions are recommended to validate these findings.

Indexed as

Antifungal AgentsFungiPlant DiseasesTrichodermaAlternariaColletotrichumFusariumPest Control, BiologicalRhizoctoniaAntifungal AgentsBiological controlDual cultureIn vitroSlide cultureTrichoderma citrinovirideVolatile and non-volatile metabolites

Identifiers

PMID41290969
PMCPMC12749251

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.