Evidence map›Paper›PMID 41290815›Full record

ArticleScientific reports2025

Major histocompatibility class II in murine antigen presenting cells is modified with a branched K63 and K11-linked ubiquitin chain.

Haiyin Liu, Lauren Francis, Laura F Dagley, Simon A Cobbold, Andrew I Webb, David Komander, Jose A Villadangos, Justine D Mintern

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Haiyin LiuDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Rd, Parkville, VIC, 3010, Australia.
Lauren FrancisDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Rd, Parkville, VIC, 3010, Australia.
Laura F DagleyAdvanced Technology and Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3010, Australia.
Simon A CobboldDepartment of Medical Biology, University of Melbourne, Melbourne, VIC, 3052, Australia.
Andrew I WebbAdvanced Technology and Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3010, Australia.
David KomanderDepartment of Medical Biology, University of Melbourne, Melbourne, VIC, 3052, Australia.
Jose A VilladangosDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Rd, Parkville, VIC, 3010, Australia. j.villadangos@unimelb.edu.au.
Justine D MinternDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Rd, Parkville, VIC, 3010, Australia. jmintern@unimelb.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Major histocompatibility class II (MHC II) is critical for adaptive immunity. MHC II intracellular trafficking and degradation is regulated by ubiquitination. Poly-ubiquitination (Ub) of MHC II directs it away from the plasma membrane and is a critical determinant of MHC II turnover. The MHC II Ub chain has not been characterized. Here, we describe the poly-Ub chain associated with MHC II in primary murine antigen presenting cells; conventional dendritic cells (cDCs) and B cells. Analysis was conducted for endogenous murine MHC II isoforms H2-A and H2-E immunoprecipitated from primary cells. We show that ubiquitination of both I-A and I-E expressed by cDCs and B cells is dependent on the E3 Ub ligase MARCH1. Using mass spectrometry Ub chain linkage analysis and innovative Ub "clipping" proteomics we comprehensively defined the features of the MHC II poly-Ub chain. This revealed the MARCH1-dependent poly-Ub chain associated with MHC II is composed of a branched chain with K11 and K63 Ub chain linkages. This is the first description of the Ub chain linkages and architecture associated with MARCH1-mediated ubiquitination of MHC II in primary antigen presenting cells. This is important because it creates possibilities for manipulation of MHC II function in adaptive immunity.

Indexed as

Antigen-Presenting CellsHistocompatibility Antigens Class IIUbiquitinAnimalsB-LymphocytesDendritic CellsMiceMice, Inbred C57BLUbiquitinationUbiquitin-Protein LigasesHistocompatibility Antigens Class IIMARCH1 protein, mouseUbiquitinUbiquitin-Protein Ligases

Identifiers

PMID41290815
PMCPMC12647164

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