Evidence map›Paper›PMID 41290642›Full record

ArticleNature communications2025

Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.

Megan A Steves, Ke Xu

Abstract read
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Megan A StevesDepartment of Chemistry, University of California, Berkeley, Berkeley, California, USA.ORCID http://orcid.org/0000-0002-1410-5650
Ke XuDepartment of Chemistry, University of California, Berkeley, Berkeley, California, USA. xuk@berkeley.edu.ORCID http://orcid.org/0000-0002-2788-194X

Funding

Intracellular transport and organelle biology at the nanoscale: A multidimensional super-resolution approachR35GM149349 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Ke Xu · 2023 to 2026
$1.8M
NIGMS NIH HHS R35 GM149349NSF | Directorate for Mathematical & Physical Sciences | Division of Chemistry (CHE) CHE-2203518U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM149349
6 · The paper itself

Abstract

Wide-field single-molecule tracking is often limited by the ~10 ms camera frame time. We introduce SpeedyTrack, which directly enables microsecond wide-field single-molecule tracking/imaging on common setups. Harnessing the native sub-microsecond vertical charge shifting capability of EM-CCDs, SpeedyTrack staggers wide-field single-molecule images along the CCD chip at ~10-row spacings between consecutive timepoints, effectively projecting the time domain to the spatial domain. Wide-field tracking is achieved for molecules diffusing at up to 1000 µm

Identifiers

PMID41290642
PMCPMC12647137

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.