Evidence map›Paper›PMID 41290627›Full record

ArticleNature communications2025

Solvent-induced partial cellular fixation decodes proteome-wide drug targets and downstream pathways in living cells.

Ting Yu, Yan Wang, Keyun Wang, Zheng Fang, Haiyang Zhu, Xiaolei Zhang, Yanan Li, Mingliang Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ting YuState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.ORCID http://orcid.org/0009-0007-7833-0455
Yan WangState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Keyun WangState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Zheng FangState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.ORCID http://orcid.org/0000-0003-2139-3256
Haiyang ZhuState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Xiaolei ZhangState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Yanan LiState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Mingliang YeState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. mingliang@dicp.ac.cn.ORCID http://orcid.org/0000-0002-5872-9326

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Comprehensive knowledge of drug targets, their binding efficacy, and the subsequent activated cellular pathways is critical for determining therapeutic efficacy, elucidating mechanisms of action, and identifying potential side effects. However, acquiring this information in living cells remains challenging using routine proteomics methods. In this study, we propose an approach, the Solvent-Induced partial Cellular Fixation Approach (SICFA), to assess protein stability and drug interactions in living cells. By combining SICFA with quantitative proteomics, we can quantify stability for approximately 5600 proteins within the intracellular proteome. SICFA demonstrates high sensitivity in detecting drug-induced stability changes in target proteins and downstream effector proteins. Time-resolved SICFA allows the tracking of drug-induced molecular events over time, revealing the early impact of 5-Fluorouracil on RNA post-transcriptional modifications and ribosome biogenesis. Unlike traditional proteomics, SICFA offers enhanced sensitivity in detecting early biochemical events, providing critical insights into drug-target interactions and the sequence of drug-induced pathway alterations.

Indexed as

ProteomeProteomicsSolventsFluorouracilHumansProtein StabilityRibosomesFluorouracilProteomeSolvents

Identifiers

PMID41290627
PMCPMC12647918

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.