Evidence map›Paper›PMID 41289997›Full record

ArticleNeuron2026

Ultrastructural membrane dynamics of mouse and human cortical synapses.

Chelsy R Eddings, Minghua Fan, Yuuta Imoto, Kie Itoh, Xiomara McDonald, Jens Eilers, William S Anderson, Paul F Worley, Kristina Lippmann, David W Nauen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Ultrafast endocytosis in mouse cortical inhibitory synapses.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Chelsy R EddingsDepartment of Cell Biology, John Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Minghua FanSolomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Yuuta ImotoDepartment of Cell Biology, John Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Kie ItohDepartment of Cell Biology, John Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Xiomara McDonaldDepartment of Cell Biology, John Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Jens EilersCarl-Ludwig-Institute of Physiology, Faculty of Medicine, Leipzig University, Leipzig 04103, Germany.
William S AndersonDepartment of Neurosurgery, John Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Paul F WorleySolomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, The Johns Hopkins Hospital, Baltimore, MD 21205, USA.
Kristina LippmannCarl-Ludwig-Institute of Physiology, Faculty of Medicine, Leipzig University, Leipzig 04103, Germany. Electronic address: kristina.lippmann@medizin.uni-leipzig.de.
David W NauenDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: dwnauen@jhmi.edu.
Shigeki WatanabeDepartment of Cell Biology, John Hopkins University School of Medicine, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD 21205, USA; The Center for Cell Dynamics, John Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: shigeki.watanabe@jhmi.edu.

Funding

TRANSCRIPTIONAL MECHANISMS OF HIPPOCAMPAL AGINGP01AG009973 · NIA · UNIVERSITY OF NORTH CAROLINA CHAPEL HILL · PI Audrey Eileen Branch, JAMES J KNIERIM · 1991 to 2026
$52.4M
NPTX2: Preserving memory circuits in normative aging and Alzheimer's DiseaseR01AG072643 · NIA · UNIVERSITY OF ARIZONA · PI BARNES, CAROL A. · 2021 to 2025
$5.7M
Ultrafast membrane trafficking at synapsesR35NS132153 · NINDS · JOHNS HOPKINS UNIVERSITY · PI Shigeki Watanabe · 2023 to 2026
$4.1M
Reviving electron microscopy for synaptic cell biologyDP2NS111133 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2018
$2.5M
Spatial and molecular determinants of fusion probability and timingR01NS105810 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2022
$1.8M
PROGRAM TO DEVELOP RESEARCH IN IMMUNOLOGY OF AGINGK07AG000997 · NIA · LOYOLA UNIVERSITY CHICAGO · PI WITTE, PAMELA L. · 2000 to 2004
$540k
Request for Leica High-pressure Freezer and Automated Freeze-substitution SystemS10RR026445 · NCRR · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2010 to 2010
$273k
NCRR NIH HHS S10 RR026445NIA NIH HHS K07 AG000997NIA NIH HHS P01 AG009973NIA NIH HHS R01 AG072643NINDS NIH HHS DP2 NS111133NINDS NIH HHS R01 NS105810NINDS NIH HHS R35 NS132153
6 · The paper itself

Abstract

Live human brain tissues provide unique opportunities for understanding synaptic transmission. Investigations have been limited to anatomy, electrophysiology, and protein localization, while crucial parameters, such as synaptic vesicle dynamics, were not visualized. Here, we utilize zap-and-freeze time-resolved electron microscopy to overcome this hurdle. First, we validate the approach with calcium imaging in acute mouse brain slices. Next, we show that synaptic vesicle endocytosis is induced in both mouse and human brain slices. Crucially, clathrin-free pits appear immediately next to the active zone, where ultrafast endocytosis normally occurs, and can be trapped at this location by a dynamin inhibitor. In both species, a protein essential for ultrafast endocytosis, dynamin 1xA, localizes to the region peripheral to the active zone, the putative endocytic zone, indicating a possible conserved mechanism between mouse and human. This approach has the potential to reveal dynamic, high-resolution information about synaptic membrane trafficking in intact human brain slices.

Indexed as

Cerebral CortexSynapsesSynaptic MembranesSynaptic VesiclesAnimalsCalciumDynaminsEndocytosisHumansMaleMiceMice, Inbred C57BLSynaptic TransmissionCalciumDynaminsdynamin 1xAhigh-pressure freezinghuman neocortexstimulated emission depletion microscopysynaptic transmissionsynaptic vesicle endocytosistime-resolved electron microscopytwo-photon calcium imagingultrafast endocytosiszap-and-freeze

Identifiers

PMID41289997
PMCPMC12703363

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.