Evidence map›Paper›PMID 41288772›Full record

ArticleMikrochimica acta2025

B-PER tandem assembly CRISPR/Cas12a cascade amplification strategy based fluorescence/colorimetric dual-mode for detection of MC-LR.

Weiqing Sun, Xinru Ren, Jiadong Huang, Yu Wang, Su Liu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Weiqing SunSchool of Water Conservancy and Environment, University of Jinan, Jinan, 250022, P.R. China.
Xinru RenSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, P.R. China.
Jiadong HuangSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P.R. China.
Yu WangSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P.R. China.
Su LiuSchool of Water Conservancy and Environment, University of Jinan, Jinan, 250022, P.R. China. stu_lius@ujn.edu.cn.

Funding

National Natural Science Foundation of China 32072330Natural Science Foundation of Shandong Province ZR2022MB066
6 · The paper itself

Abstract

Microcystins are the most common, most powerful and most toxic types of cyanobacteria, which seriously threaten the public health and ecological environment. Biosensor has been widely used in Microcystin-Leucine-Arginine (MC-LR) detection. In this study, we report an efficient single-hairpin with double primers (the trigger chain T hybridizes with the left end a, and the amplified product ab hybridizes with the right end a) for bidirectional PER (B-PER) amplification strategy for uniform visual and fluorescence detection of trace amounts of MC-LR in the environment using quantum dots (QDs) as signal reporters. The biosensor is triggered by a single stranded DNA of MC-LR specificity to initiate the amplification by B-PER. From the long product of the B-PER, the trans-cleavage activity of CRISPR/Cas12a is activated, and the Ag

Indexed as

Biosensing TechniquesColorimetryCRISPR-Cas SystemsMicrocystinsNucleic Acid Amplification TechniquesCadmium CompoundsFluorescenceLimit of DetectionMarine ToxinsQuantum DotsSpectrometry, FluorescenceCadmium Compoundscyanoginosin LRMarine ToxinsMicrocystinsBiosensorB-PERCdTe QDsCRISPR/Cas12aFluorescence quenchingMC-LRVisual detection

Identifiers

PMID41288772

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.