mRNA caps accumulate in stress granules and are essential for their formation.

Mingxin Guo, Ying Feng, Guantong Qi, Wenran Ma, Xiaoli He, Ruiqi Li, Yu Liu, Jin Wang

Abstract read

In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Mingxin GuoState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.ORCID http://orcid.org/0000-0003-3697-5064

Ying FengState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.

Guantong QiState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.

Wenran MaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.

Xiaoli HeState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.

Ruiqi LiState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.

Yu LiuState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China. yuliu@imu.edu.cn.

Jin WangState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China. jinwang@imu.edu.cn.ORCID http://orcid.org/0000-0001-5511-3983

Funding

Central Guiding Fund for Local Science and Technology Development 2020ZY0100Inner Mongolia Collaborate Innovation Cultivate Center for Castor 2021JQ03Inner Mongolia Collaborate Innovation Cultivate Center for Castor 2021PT0002Innovative Research Group Project of the National Natural Science Foundation of China 31960140Innovative Research Group Project of the National Natural Science Foundation of China 32360147Natural Science Foundation for Young Scientists of Shanxi Province 2022QN02013State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry SKL-IT-201830Ten Thousand Talent Plans for Young Top-notch Talents of Yunnan Province Ten Thousand Talent Plans for Young Top-notch Talents of Yunnan Province

6 · The paper itself

Abstract

Stress granules (SGs) are essential cytoplasmic, membraneless organelles that form in response to cellular stress, functioning to prevent mRNA translation and protect mRNA from damage. However, the mechanism of SG formation remains largely unknown. Here, utilizing a systems-level technique for quantification of RNA cap epitranscriptome, we found that mRNA Cap1 and non-canonical caps are predominantly enriched in SGs, with the composition of mRNA caps in SGs of mammalian cells differing between different stress conditions. Knockdown of RNA guanine-7-methyltransferase (RNMT) and phosphorylated CTD interacting factor 1(PCIF1) both resulted in substantial changes in the content and composition of mRNA caps and RNMT knockdown caused failure of SG formation under different stress conditions. Furthermore, proteomic, Co-IP and confocal immunofluorescence analysis of these knockdown cells and SGs revealed that mRNAs partition into SGs through cap-protein interactions. These findings collectively revealed the significant role of mRNA cap in SG formation and stress response in mammalian cells.

Indexed as

Cytoplasmic GranulesRNA CapsRNA, MessengerStress GranulesStress, PhysiologicalAnimalsGene Knockdown TechniquesHeLa CellsHumansMethyltransferasesMethyltransferasesRNA CapsRNA, MessengerCapQuantmRNA capStressStress granule

Identifiers

PMID41288727

DOI10.1007/s00018-025-05947-8

PMCPMC12647448

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.