ArticleCurrent protocols2025
Humanizing Mice for Human Immunodeficiency Virus Infection Studies.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Nef as a driver of immunodeficiency and HIV-associated diseases: insights from mouse models.Frontiers in immunology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
An estimated 40 million people are infected with human immunodeficiency virus type 1 (HIV-1) and there is no effective cure. Humanized mice are a useful model to study transmission, pathogenesis, prevention, and treatment of HIV-1, which only infects human cells. Here we provide detailed protocols to humanize immunodeficient mice, e.g., NSG or MISTRG6 strains, using human hematopoietic stem cells isolated from cord blood and a flow cytometry panel of antibodies to assess human immune cell reconstitution. Furthermore, we describe how to produce, concentrate, and titer HIV-1 in vitro, which can be used to challenge humanized mice either systemically or mucosally. Finally, we adapted a sensitive quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay with internal control to detect HIV-1 RNA in longitudinal plasma samples to measure viremia over time in the mice. © 2025 Wiley Periodicals LLC. Basic Protocol 1: Isolation of CD34+ cells from human cord blood Basic Protocol 2: Injection of human CD34+ cells in immunodeficient mice Basic Protocol 3: Flow cytometry of peripheral blood mononuclear cells from mouse blood Basic Protocol 4: Production and titration of HIV-1 Basic Protocol 5: Systemic HIV-1 challenge of humanized mice Alternate Protocol: Mucosal HIV-1 challenge of humanized mice Basic Protocol 6: Single-copy HIV-1 RNA detection in mouse plasma Support Protocol 1: Retrovirus internal control for plasma virus isolation and RT-qPCR Support Protocol 2: Production of HIV-1 standard for RT-qPCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.