ArticleParasites & vectors2025
Surveillance of pyrethroid resistance and detection of voltage-gated sodium channel gene mutations in Aedes albopictus populations from Shanghai, China.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundAs a global hub for trade and tourism, Shanghai faces escalating risks of dengue fever due to imported and local transmission. Aedes albopictus, the primary dengue vector in the region, is predominantly controlled using pyrethroid insecticides. However, widespread resistance threatens their efficacy.
methodsResistance levels of Ae. albopictus to four pyrethroids (deltamethrin, permethrin, lambda-cyhalothrin, beta-cypermethrin) in 12 Shanghai districts were assessed via adult mosquito contact tube bioassays, with resistance categorized by mortality rates at diagnostic doses. Voltage-gated sodium channel (VGSC) gene mutations in 255 adult mosquitoes were identified through sequencing. A loop-mediated isothermal amplification (LAMP) assay was developed to rapidly detect the F1534S mutation in the VGSC gene.
resultsThe Baoshan district population exhibited resistance to cyhalothrin (mortality: 80.3%), while populations in all other districts displayed confirmed resistance (mortality < 80%) to all four pyrethroids. VGSC mutation analysis revealed high frequencies of F1534S (84.71%), moderate V1016G (21.96%), and rare I1532T (1.57%). An optimized LAMP assay for F1534S detection was established with the following parameters: 0.5 U/μl Bst DNA polymerase, 0.8 mM deoxynucleoside triphosphates (dNTPs), 3.00 mM Mg
conclusionsPyrethroid resistance is pervasive in Shanghai's Ae. albopictus populations, driven by high-frequency VGSC mutations. The LAMP assay provides a rapid field-deployable tool for resistance monitoring, guiding targeted vector control strategies.
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