ArticleParasites & vectors2025
Development and application of a PCR-RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
backgroundAedes albopictus is a primary vector of multiple arboviruses, including dengue, chikungunya, yellow fever, and Zika virus. Its control relies heavily on pyrethroid insecticides. The V1016G mutation in the voltage-gated sodium channel (VGSC) is a well-documented mechanism conferring pyrethroid resistance in Ae. albopictus, which directly challenges the efficacy of pyrethroid-based control. Understanding of the status of insecticide resistance will offer insights to inform evidence-based vector management. However, current phenotypic monitoring is laborious and time-consuming, highlighting the need for rapid and reliable genotyping tools.
methodsTo detect the V1016G mutation, we developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. This assay was then applied to genotype 208 field-collected Ae. albopictus mosquitoes. These samples were collected in 2024 from seven counties/districts within Guangyuan City, a prefecture in northern Sichuan, China.
resultsThe PCR-RFLP assay demonstrated 100% concordance with Sanger sequencing results. Genotyping confirmed the widespread presence of the 1016G allele, with frequencies ranging from 3.13% to 14.06%. The resistance allele (1016G) was exclusively detected in heterozygotes, and all populations conformed to Hardy-Weinberg equilibrium (P > 0.05). Furthermore, no significant temporal changes in allele frequencies were detected between 2020 and 2024 across the populations (P > 0.05).
conclusionsThis study established a cost-effective and reliable PCR-RFLP assay for detecting the V1016G mutation in Ae. albopictus, and demonstrated the widespread distribution of this mutation across Guangyuan City, Sichuan Province of China.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.