Evidence map›Paper›PMID 41286603›Full record

ArticleBMC plant biology2025

Genome-wide development of genomic SSR markers, polymorphism analysis, and dosage-aware SSR fingerprinting in hexaploid Camellia Oleifera.

Chengcai Zhang, Ping Lin, Xiaohua Yao, Huadong Ren, Kailiang Wang

Abstract read
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Genetic Diversity and Population Structure ofPlants (Basel, Switzerland) · 2025
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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Chengcai ZhangState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China.
Ping LinState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China.
Xiaohua YaoState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China.
Huadong RenState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China. renhd@163.com.
Kailiang WangState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China. wangkl163@163.com.

Funding

Special Investigation on Basic Resources of Science and Technology 2019FY100801
6 · The paper itself

Abstract

backgroundOil-Camellia is an important woody oil crop, and most cultivated cultivars are hexaploid. Unresolved kinship among major cultivars and the lack of reliable identification methods have hindered both the development of new cultivars and the deployment of elite ones. An important reason is the limited availability of molecular markers suitable for genetic analysis.

resultsUsing five published oil-Camellia genomes, we surveyed genome-wide SSRs and found densities of one SSR per 4.22–4.67 kb across species. Based on the C. oleifera var. Nanyongensis reference genome, we designed 14,854 primer pairs and validated 132 new SSR markers. Genetic diversity and relationships among 44 hexaploid cultivars were assessed with a 30-marker subset, yielding 3–21 alleles per locus; polymorphism information content ranged from 0.07 to 0.91. Phylogenetic analyses using dosage-resolved SSR genotypes revealed the genetic relationships between 44 cultivars. We evaluated the discriminatory power of each marker and identified a minimal set (Coz09127 and Coz14197) that distinguishes all cultivars; using these two core markers, we constructed—for the first time—fingerprints for 44 hexaploid C. oleifera cultivars.

conclusionsWe surveyed SSR distribution in oil-Camellia genomes and developed 132 SSR markers. Using 30 loci, we characterized genetic diversity and relationships among 44 hexaploid cultivars, and identified a minimal two-marker set that distinguishes all tested cultivars, enabling construction of cultivar DA-SSR fingerprints. These results strengthen the genetic toolkit for polyploid C. oleifera and support reliable cultivar identification and deployment in breeding and production.

Indexed as

CamelliaGenome, PlantMicrosatellite RepeatsPolymorphism, GeneticPolyploidyDNA FingerprintingGenetic MarkersPhylogenyGenetic MarkersCultivar identificationFingerprintGenetic diversityOil-CamelliaPolymorphism

Identifiers

PMID41286603
PMCPMC12642055

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