Evidence map›Paper›PMID 41286547›Full record

ArticleScientific reports2025

A noninvasive test for human prion disease using hair roots and scalp.

Thi-Thu-Trang Dong, Hiroyuki Honda, Akio Akagi, Yasushi Iwasaki, Hitaru Kishida, Tadashi Tsukamoto, Kensaku Kasuga, Hirotsugu Takashima, Tatsuhiro Terada, Kensuke Ikenaka and 20 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors.

Thi-Thu-Trang DongDepartment of Acute Diseases and Emergency - Senior Official Institute, 108 Military Central Hospital, Hanoi, Vietnam.
Hiroyuki HondaDepartment of Neurology, Neuropathology Center, National Hospital Organization, Omuta National Hospital, Omuta, Japan.
Akio AkagiDepartment of Neuropathology, Institute for Medical Science of Aging, Aichi Medical University, Nagakute, Japan.
Yasushi IwasakiDepartment of Neuropathology, Institute for Medical Science of Aging, Aichi Medical University, Nagakute, Japan.
Hitaru KishidaDepartment of Neurology, Yokohama City University Medical Center, Yokohama, Japan.
Tadashi TsukamotoResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Kensaku KasugaJapan Prion Disease Surveillance Committee (J-PrD-SC), Tokyo, Japan.
Hirotsugu TakashimaDepartment of Neurology, National Epilepsy Center, NHO Shizuoka Institute of Epilepsy and Neurological Disorders (NEC), Shizuoka, Japan.
Tatsuhiro TeradaDepartment of Neurology, National Epilepsy Center, NHO Shizuoka Institute of Epilepsy and Neurological Disorders (NEC), Shizuoka, Japan.
Kensuke IkenakaJapan Prion Disease Surveillance Committee (J-PrD-SC), Tokyo, Japan.
Takeshi IkeuchiDepartment of Molecular Genetics, Brain Research Institute, Niigata University, Niigata, Japan.
Tsuyoshi MoriDivision of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Hideki MochizukiJapan Prion Disease Surveillance Committee (J-PrD-SC), Tokyo, Japan.
Kenjiro OnoDepartment of Neurology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.
Yoshihisa TakiyamaDepartment of Neurology, Fuefuki Central Hospital, Fuefuki, Japan.
Tsuyoshi HamaguchiResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Hiroyuki MurotaDepartment of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Nobuo SanjoResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Takeshi FujimotoDepartment of Neurology, Sasebo City General Hospital, Sasebo, Japan.
Michio KitayamaDepartment of Internal Medicine Kawasaki Medical School General Medical Center, Okayama, Japan.
Koji FujitaJapan Prion Disease Surveillance Committee (J-PrD-SC), Tokyo, Japan.
Motohiro YukitakeResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Shinsuke FujiokaDepartment of Pharmaceutical Sciences, International University of Health and Welfare, Okawa, Japan.
Noriyuki NishidaDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Yoshio TsuboiResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Tetsuyuki KitamotoResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Masaki TakaoResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Masahito YamadaResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Hidehiro MizusawaResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan.
Katsuya SatohResearch Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Tokyo, Japan. satoh-prion@nagasaki-u.ac.jp.

Funding

Japan Agency for Medical Research and Development (AMED) 20ek0109362Ministry of Health, Labour and Welfare of Japan KSat No. 14507303Research Committee of Prion Disease and Slow Virus Infection, Research on Policy Planning and Evaluation for Rare and Intractable Diseases, Health and Labour Sciences Research JPMH20FC1054Research Committee of Surveillance and Infection Control of Prion Disease, the Ministry of Health, Labour, and Welfare of Japan JPMH20FC2001
6 · The paper itself

Abstract

Invasive tests like cerebrospinal fluid (CSF) examination are highly effective for diagnosing human prion disease (HPD). Real-time quaking-induced conversion (RT-QuIC) CSF assay demonstrates 80-90% sensitivity for HPD diagnosis. To establish a minimally invasive diagnostic approach, we evaluated RT-QuIC testing on hair root and scalp samples. We collected scalp samples from 22 HPD and 5 non-HPD patients during pathological examinations and analyzed them using RT-QuIC assay and neuropathological methods. In our prospective study, hair root and CSF samples from 300 patients were tested using RT-QuIC assay along with other biomarkers, including 14-3-3 protein, total tau protein, RT-QuIC CSF assay, and MRI findings. All 22 HPD patients demonstrated positive prion seeding activity in scalp and hair root RT-QuIC assays. Neuropathological examination in one HPD patient revealed abnormal prion protein in scalp tissue. Among 177 HPD patients diagnosed by Japan Prion Surveillance Committee and 123 non-HPD patients, RT-QuIC assay of hair roots demonstrated sensitivity and specificity of 45.8% and 100%, respectively. Sensitivities of 14-3-3 protein, total tau protein, RT-QuIC CSF assay, and MRI findings were 83.1%, 86.4%, 74.6%, and 100%, respectively, with specificities of 65.0%, 65.0%, 100%, and 56.1%, respectively. RT-QuIC assays could be developed into novel diagnostic methods for neurodegenerative diseases.

Indexed as

HairPrion DiseasesScalp14-3-3 ProteinsAdultAgedAged, 80 and overBiomarkersFemaleHumansMagnetic Resonance ImagingMaleMiddle AgedProspective StudiesSensitivity and Specificitytau Proteins14-3-3 ProteinsBiomarkerstau ProteinsBiomarkersHair rootPrion diseaseRT-QuIC assayScalp

Identifiers

PMID41286547
PMCPMC12644664

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