Evidence map›Paper›PMID 41285221›Full record

ArticleBiochimie2026

A Trichomonas vaginalis C2-XYPPX-repeat protein with a structured C2 domain displaying dampened flexibility upon binding calcium.

Garry W Buchko, Lijun Liu, Kevin P Battaile, Justin K Craig, Elizabeth K Harmon, Wesley C Van Voorhis, Peter J Myler, Scott Lovell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimie, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Garry W BuchkoSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA; School of Molecular Biosciences, Washington State University, Pullman, WA, 99164, USA. Electronic address: garry.buchko@pnnl.gov.
Lijun LiuSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Protein Structure & X-Ray Crystallography Laboratory, University of Kansas, Lawrence, KS, 66047, USA.
Kevin P BattaileNew York Structural Biology Center, New York, NY, 10065, USA.
Justin K CraigSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, 98195, USA.
Elizabeth K HarmonSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, 98195, USA.
Wesley C Van VoorhisSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, 98195, USA; Department of Microbiology, University of Washington School of Medicine, Seattle, WA, 98195, USA; Department of Global Health, University of Washington, Seattle, WA, 98105, USA.
Peter J MylerSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Seattle Children's Research Institute, Seattle, WA, 98101, USA; Department of Pediatrics, University of Washington, Seattle, WA, 98195, USA; Department of Medical Education and Biomedical Informatics, University of Washington, Seattle, WA, 98195, USA.
Scott LovellSeattle Structural Genomics Center for Infectious Disease, Seattle, WA, USA; Protein Structure & X-Ray Crystallography Laboratory, University of Kansas, Lawrence, KS, 66047, USA.

Funding

Centers for Research on Structural Biology of Infectious Diseases: Universal Influenza Vaccine Research75N93022C00036 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI STAKER, BART · 2022 to 2025
$21.7M
An Eiger2 XE 9M detector for the NYSBC-operated NYX beamline at NSLS-IIS10OD030394 · OD · NEW YORK STRUCTURAL BIOLOGY CENTER · PI BATTAILE, KEVIN P · 2021 to 2021
$1.8M
NIH HHS 75N93022C00036NIH HHS S10 OD030394
6 · The paper itself

Abstract

C2 domains are ubiquitous membrane-binding modules of ∼130 residues in eukaryotes that are often associated with proteins involved in membrane trafficking and lipid modification. The genome of Trichomonas vaginalis, the most common, non-viral, sexually transmitted human pathogen, encodes eight genes that contain a N-terminal C2 module linked to a XYPPX-repeat domain of more than four XYPPX repeats (C2-XYPPX). While the function of the XYPPX-repeat domain remains unknown, its multiple association with C2 domains in T. vaginalis suggests it is important. The C2 domain from one of these C2-XYPPX-repeat proteins, Tv-C2-1, was structurally and physically characterized using X-ray crystallography and NMR spectroscopy. The crystal structure for Tv-C2-1 shows that this domain shares a fold common to all C2 domains, a compact Greek-key motif composed of eight anti-parallel β-strands in the type-2 topology. An NMR chemical shift perturbation study with Ca

Indexed as

CalciumProtozoan ProteinsTrichomonas vaginalisAmino Acid SequenceC2 DomainsCrystallography, X-RayModels, MolecularProtein BindingCalciumProtozoan Proteins

Identifiers

PMID41285221
PMCPMC13506139

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.