ArticleJournal of visualized experiments : JoVE2025
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses.
Article in Journal of visualized experiments : JoVE, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- A multiplex serological assay for quantification of arbovirus antibody kinetics and neutralizing antibodies.PLoS neglected tropical diseases · 2026Article
- The national burden from O'nyong'nyong and chikungunya viruses in Cameroon.medRxiv : the preprint server for health sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Arthropod borne viruses (arboviruses) are a group of mosquito-borne pathogens that are globally distributed. Since the 2016 outbreak of Zika virus (ZIKV) in the Americas that brought these infections to public awareness, arboviruses continue to impose a considerable public health burden. Their emergence in previously non-endemic regions is a cause of public health concern. The surveillance and control of arboviral infections is a current priority for the World Health Organization (WHO), which launched a global strategic plan to fight arboviral diseases in 2024. The multiplex® technology has revolutionized the field of serological surveillance, allowing simultaneous measurement of multiple antibodies while requiring minimum sample volume. Building on expertise in the development of multiplex serological tests, a bead-based multiplex assay for the detection of antibodies to arbovirus antigens was developed. The assay comprises multiple antigens specific to common arboviruses, including dengue (DENV), yellow fever (YFV), Zika, and chikungunya (CHIKV) viruses. A semi-automated method was used to covalently bind antigens to microsphere beads. This new protocol is highly effective, enabling the coupling of up to 96 antigens in a single experiment while ensuring reproducible results. Median Fluorescence Intensity (MFI) data were acquired using a user-friendly fluorescence imaging system.When applied to human serum samples from endemic countries, we anticipate that this assay will constitute a powerful tool for sero-surveillance efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.