ArticleFish physiology and biochemistry2025
Central administration of docosahexaenoic acid, eicosapentaenoic acid, or linoleic acid activates hypothalamic fatty acid sensing and is involved in reproductive regulation in spotted scat (Scatophagus argus).
Article in Fish physiology and biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
The binding of fatty acid (FA) to fatty acid translocase (FAT/CD36) and subsequent regulation of lipid metabolism transcription factors, such as peroxisome proliferator-activated receptors α (PPARα) and sterol regulatory element-binding protein-1c (SREBP-1c), is an important hypothalamic fatty acid-sensing mechanism in vertebrates. The hypothalamus exhibits responsiveness to specific fatty acids and is involved in reproductive regulation. However, the link between FA sensing and reproductive regulation in the hypothalamus remains poorly understood teleosts. Therefore, the expression of genes involved in the FA-sensing mechanism-such as fat/cd36, pparα, and srebp1c, as well as gonadotropin-releasing hormone (GnRH: cgnrh, sgnrh, sbgnrh) in the hypothalamus-was investigated after intracranial administration and hypothalamic incubation of polyunsaturated fatty acids (PUFAs), including docosahexaenoic acid (DHA), eicosapentanoic acid (EPA), and linoleic acid (LA) for 4 h in the spotted scat (Scatophagus argus). Compared to the control, intracranial administration and hypothalamic incubation of DHA and LA, as well as hypothalamic incubation of EPA, increased the levels of fat/cd36, pparα, and srebp1c. However, the expression of fat/cd36 and pparα was significantly decreased, and the level of srebp1c showed a downward trend only in the EPA group when co-incubated with the FAT/CD36 inhibitor Sulfo-N-succinimidyl oleate sodium (SSO), compared to incubation with EPA alone. After the application of PPARα antagonist GW6471, the expression of pparα in the hypothalamus decreased significantly in the DHA, EPA, and LA treatment groups, compared to incubation with DHA, EPA, and LA alone. Levels of fsh and lh in the pituitary gland were changed significantly after the intracranial administration of DHA, EPA, and LA. Compared to the control, the levels of cgnrh, sgnrh, and sbgnrh increased in the hypothalamus after both hypothalamic incubation and intracranial administration of EPA and LA. Levels of cgnrh increased in the hypothalamus after incubation, but not after intracranial administration of DHA. However, compared to the control, levels of sgnrh and sbgnrh increased after hypothalamic incubation with DHA and EPA. Only sgnrh levels decreased after incubation with LA in the presence of SSO compared to the FA treatment group alone. In the presence of GW6471, only sgnrh levels decreased after incubation with LA in the presence of SSO compared to the FA treatment group alone; the levels of cgnrh, sgnrh, and sbgnrh after LA incubation, the levels of sbgnrh after EPA incubation, and the levels of sbgnrh after DHA incubation were all significantly decreased compared to the control. These results showed that DHA, EPA, and LA could activate fat/cd36 and pparα, which are involved in reproductive regulation in the hypothalamus of the spotted scat. These results provide evidence that hypothalamic FA sensing is involved in regulating reproduction in teleosts.
Indexed as
Identifiers
41284092What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.