ArticlePediatric gastroenterology, hepatology & nutrition2025
Next Generation Sequencing in Defining the Composition of Bacterial Microbiota of Donated Human Milk.
Article in Pediatric gastroenterology, hepatology & nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Purpose: Human milk microbiota can significantly influence children's health. This study aimed to investigate the composition and diversity of the bacterial microbiota in donated human milk (DHM) and analyze the possible influencing factors. Methods: Archival samples from single-donor pools of raw DHM expressed from 88 different donors within the first six months postpartum were included. Donor-, infant-, and milk-related data were collected and analyzed using descriptive and comparative statistical methods. The hypervariable region V1-V3 of the bacterial 16S ribosomal ribonucleic acid gene was sequenced using the Illumina MiSeq platform to characterize the microbial profile of the donated milk. Results: Conclusion: This study provides evidence of the potential influence of donor infant-related factors and milk characteristics on the composition and alpha diversity of bacterial microbiota in DHM. Notably, previous studies utilizing high-throughput sequencing technology to examine the human milk microbiota have not focused on pooled DHM samples.
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