Evidence map›Paper›PMID 41282104›Full record

ArticleResearch square2025

Piezo1-mediated mechano-energetics regulate CAR T cell function.

Ngoc Luu, Rui Li, Yifei Fang, Huishu Wang, Yujing Song, Ruiqi Chen, Xiangyi Fang, Junru Liao, Tracy Chen, Andre Kelly and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ngoc LuuDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Rui LiDepartment of Mechanical and Aerospace Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Yifei FangDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Huishu WangDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Yujing SongDepartment of Mechanical and Aerospace Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Ruiqi ChenDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Xiangyi FangCenter for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
Junru LiaoDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Tracy ChenDepartment of Mechanical and Aerospace Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Andre KellyCenter for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
Alexander A ShestovCenter for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
Katsuo KurabayashiDepartment of Mechanical and Aerospace Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.
Roddy O'ConnorCenter for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
Louis HodgsonDepartment of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.ORCID 0000-0001-9188-7580
Saba GhassemiCenter for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
Weiqiang ChenDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, NY 11201, USA.ORCID 0000-0002-9469-8328

Funding

Vaccine FacilityP30CA016087 · NCI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI MARK Reid PHILIPS · 1985 to 2026
$83.1M
Multiplex Imaging of Signaling Pathways in Cell MotilityR35GM136226 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI Louis Hodgson · 2020 to 2026
$5.3M
Increasing the efficacy of non-activated CAR T cells by modulating IFN1 signalingR01CA292680 · NCI · UNIVERSITY OF PENNSYLVANIA · PI Saba Ghassemi · 2024 to 2026
$2.9M
Purchase of spinning disk confocal microscopeR35GM133646 · NIGMS · NEW YORK UNIVERSITY · PI CHEN, WEIQIANG · 2019 to 2023
$2.0M
What fuels CAR T-cell serial killingR01CA278837 · NCI · UNIVERSITY OF PENNSYLVANIA · PI Roderick O'Connor · 2023 to 2026
$1.3M
Nanoplasmonic Spatiotemporal Imaging of Single-Cell Protein Secretion and Intercellular CommunicationR21GM151528 · NIGMS · NEW YORK UNIVERSITY · PI KURABAYASHI, KATSUO · 2023 to 2024
$448k
NCI NIH HHS P30 CA016087NCI NIH HHS R01 CA278837NCI NIH HHS R01 CA292680NIGMS NIH HHS R21 GM151528NIGMS NIH HHS R35 GM133646NIGMS NIH HHS R35 GM136226
6 · The paper itself

Abstract

CAR T cell cytotoxicity requires generating immense mechanical force, but the energetic costs of this process remain poorly defined. While metabolic reprogramming fuels effector function, its mechanistic connection to mechanotransduction remains unclear. By directly measuring the synaptic force and mechanical energy of single CAR T cells and linking them to their metabolic state, we proved that the mechano-energetic efficiency is a fundamental determinant of cytotoxic potency. We discovered that the mechanosensitive ion channel Piezo1 couples cytoskeletal dynamics to metabolic rewiring via Ca

Identifiers

PMID41282104
PMCPMC12633482

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.