Evidence map›Paper›PMID 41281990›Full record

ArticleVirusdisease2025

Molecular characterisation of adenoviruses in sewage and farm waste water samples in Punjab, India.

Asima Zehra, Simranpreet Kaur

Abstract read
In one paragraph

Article in Virusdisease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Asima ZehraCentre of One Health, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India.ORCID 0000-0003-1896-6535
Simranpreet KaurCentre of One Health, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to investigate the occurrence and genotype distribution of Human adenovirus (HAdV), Bovine adenovirus (BAdV), and Porcine adenovirus (PAdV) in environmental waters, with a focus on their association with water contamination and potential use in microbial source tracking in Punjab, Northern India. A total of 52 samples were collected from three different river stretches. Using nested PCR, BAdVs were detected in 16% of the samples, while HAdVs and PAdVs were found in 32 and 11% of the samples, respectively. The isolated serotypes included HAdV type 40/41, BAdV type 8/6/5/4//2, and PAdV type 3/5, with HAdV-41 being the most prevalent. Notably, multiple BAdV asymptomatic infections were observed among herds, although their implications for livestock health remain uncertain. BAdV-8 and BAdV-6, the predominant types identified, have been associated with specific clinical conditions in Punjab. Phylogenetic analysis of PAdV indicated a high nucleotide sequence identity (95.4 to 100%) among viruses detected in wastewater samples. This study represents the first investigation into the epidemiology and molecular characterisation of PAdV in porcine farm wastewater in India. Additionally, evidence of recombination among livestock adenoviruses was found, highlighting the need for continued genetic surveillance of animal AdV. These findings underscore the importance of considering BAdV and PAdV detection in fecal source attribution in environmental samples. Overall, this study provides valuable insights into the occurance, diversity, and genetic characteristics of adenoviruses in agricultural and environmental settings.

Indexed as

AdenovirusesNested-PCRPhylogeneticsqPCRSequencingSerotypes

Identifiers

PMID41281990
PMCPMC12634946

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.