ArticleJournal of biomedical optics2025
Red-shifted excitation enhances the sensitivity of red genetically encoded Ca
Article in Journal of biomedical optics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
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8 authors.
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Abstract
Significance: Two-photon (2P) holographic optophysiology, which combines optogenetic actuators and genetically encoded Aim: To eliminate crosstalk in 2P holographic optophysiology, we employed the inverse combination, namely red GECIs and blue-light-activated actuators and optimized the excitation wavelength, as conventional 2P excitation failed to detect optogenetically induced GECI responses. Approach: PC12h cells expressing various combinations of GECIs and optogenetic actuators were subjected to simultaneous Results: Under 1P excitation, crosstalk was evident in the GCaMP6m (blue)/ChRmine (red) pair, but negligible in the R-CaMP1.07 (red)/eTsChR (blue) pair. Under 2P excitation, R-CaMP1.07 showed significantly enhanced sensitivity at a red-shifted wavelength ( Conclusion: Red-shifted excitation was essential for detecting the small
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