ArticleJournal of mass spectrometry and advances in the clinical lab2025
Quantification of eight clinically relevant serum adrenal steroids in infants by UHPLC-APCI-MS/MS.
Article in Journal of mass spectrometry and advances in the clinical lab, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background and aims: There is growing interest in replacing steroid immunoassays with liquid chromatography-tandem mass spectrometry-based methods in many clinical and research applications. The aim of the present work is to develop and validate an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based method using atmospheric pressure chemical ionisation (APCI) to quantify cortisol, androstenedione, testosterone, pregnenolone, progesterone, 17-hydroxyprogesterone, 17-hydroxypregnenolone, and dehydroepiandrosterone in paediatric serum samples. Methods: Sample preparation was performed by protein precipitation using 100 µL of sample Results: The LLOQs were lower than 8 nmol/L; within- and between-run CVs were <12 %. Accuracy, in terms of recovery, was 89-111 %. Serum cortisol levels varied widely in healthy infants, and only testosterone levels exhibited sexual dimorphism ( Conclusion: A novel, accurate, and sensitive UHPLC-APCI-MS/MS-based method for the simultaneous analysis of eight steroids in serum was developed, validated and successfully applied to infants younger than three months of age. The method enabled detection of abnormal values of precursors and steroid hormones in patients with adrenal disorders, with the potential for a large impact on paediatric patient care.
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