Evidence map›Paper›PMID 41280139›Full record

ArticleIBRO neuroscience reports2025

Evaluation of an antibody panel for alpha-synuclein detection in FFPE rectal biopsies in Parkinson's disease.

Katja Schröder, Carmen Kintrup, Kristína Kulcsárová, Sebastian Heinzel, Annika Kluge, Thilo Wedel, Martina Böttner, Ralph Lucius, Manuela Pendziwiat, Stephan Schoch and 4 more

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Article in IBRO neuroscience reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Katja SchröderInstitute of Anatomy, Kiel University, Kiel, Germany.
Carmen KintrupInstitute of Anatomy, Kiel University, Kiel, Germany.
Kristína KulcsárováDepartment of Neurology, P.J. Safarik University, Kosice, Slovak Republic.
Sebastian HeinzelDepartment of Neurology, Kiel University, University Medical Center Schleswig-Holstein, Kiel, Germany.
Annika KlugeDepartment of Neurology, Kiel University, University Medical Center Schleswig-Holstein, Kiel, Germany.
Thilo WedelInstitute of Anatomy, Kiel University, Kiel, Germany.
Martina BöttnerInstitute of Anatomy, Kiel University, Kiel, Germany.
Ralph LuciusInstitute of Anatomy, Kiel University, Kiel, Germany.
Manuela PendziwiatInstitute of Clinical Molecular Biology, Kiel University, Kiel, Germany.
Stephan SchochInterdisciplinary Endoscopy, Medical Department I, Kiel University, University Medical Center Schleswig-Holstein, Kiel, Germany.
Mark EllrichmannDepartment of Internal Medicine, Gastroenterology, Hepatology, Endocrinology and Nephrology, University hospital Oldenburg, Oldenburg, Germany.
Daniela BergDepartment of Neurology, Kiel University, University Medical Center Schleswig-Holstein, Kiel, Germany.
Eva SchaefferDepartment of Neurology, Kiel University, University Medical Center Schleswig-Holstein, Kiel, Germany.
François CossaisInstitute of Anatomy, Kiel University, Kiel, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Conflicting results have been published regarding the accumulation of alpha-synuclein (aSyn) pathology, in intestinal tissues of patients with Parkinson's disease (PD). This study investigates the use of a panel of aSyn antibodies for detecting pathological aSyn in rectal biopsy samples from PD patients and healthy individuals. Materials and methods: A panel of antibodies targeting native, phosphorylated and conformation-specific forms of aSyn was used to characterize aSyn distribution in the substantia nigra and colonic tissues of controls and individuals with Lewy pathology. Distribution of aSyn was further analyzed in formalin-fixed and paraffin-embedded (FFPE) submucosal rectal biopsies of 24 PD patients and 20 healthy controls. Results: All tested antibodies valuably detected aSyn pathology in the PD substantia nigra. Native aSyn was observed at high levels in colonic tissue. The immunoreactive patterns of native aSyn, conformation-specific or phosphorylated aSyn in rectal biopsies did not show notable differences between PD patients and healthy subjects. Conclusion: The utility of immunohistochemical detection with currently commercially available antibodies for native and pathological forms of aSyn in rectal tissues appears limited. The findings advocate for the development of alternative methods to detect pathological aSyn conformers in rectal FFPE biopsies.

Indexed as

Alpha-synucleinEnteric Nervous SystemGut Brain AxisParkinson’s Disease

Identifiers

PMID41280139
PMCPMC12634310

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