Evidence map›Paper›PMID 41279865›Full record

ArticlebioRxiv : the preprint server for biology2025

Imaging Cellular Metabolic Rewiring with SuMMIT-SRS.

Yajuan Li, Zhi Li, Yuhan Li, Kevin Rhine, Sural Ranamukhaarachchi, Shuo Qin, Hongje Jang, Jorge Villazon, Zhiliang Bai, Stephanie I Fraley and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yajuan LiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Zhi LiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Yuhan LiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Kevin RhineDepartment of Cellular & Molecular Medicine, University of California San Diego, La Jolla, CA, USA.
Sural RanamukhaarachchiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Shuo QinShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Hongje JangShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Jorge VillazonShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Zhiliang BaiDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06520, USA.
Stephanie I FraleyShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Gene W YeoDepartment of Cellular & Molecular Medicine, University of California San Diego, La Jolla, CA, USA.
Rong FanDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06520, USA.
Lingyan ShiShu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Funding

HIPC Data Coordinating CenterU01AI167892 · NIAID · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI Steven H. Kleinstein, Bjoern Peters · 2022 to 2026
$18.7M
The Cancer Cell Map Initiative v2.0U54CA274502 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Emma Lundberg · 2022 to 2026
$14.2M
Functional RNA elements in the human genomeR01HG004659 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett, Eugene Wei-Ming Yeo · 2008 to 2026
$12.4M
The Human Lung BioMolecular Multi-Scale Atlas Program (HuBMAP-Lung)U54HL165443 · NHLBI · UNIVERSITY OF ROCHESTER · PI PRYHUBER, GLORIA S · 2022 to 2025
$8.0M
Kidney single cell and spatial molecular atlas project - KIDSSMAPU54DK134301 · NIDDK · WASHINGTON UNIVERSITY · PI ASHKAR, TAREK MAURICE, JAIN, SANJAY · 2022 to 2025
$7.8M
Yale TMC for Cellular Senescence in Lymphoid OrgansU54AG076043 · NIA · YALE UNIVERSITY · PI FAN, RONG, HALENE, STEPHANIE · 2021 to 2025
$7.0M
Yale Murine-TMC on Immune Cell Senescence Derived InflammationU54AG079759 · NIA · YALE UNIVERSITY · PI DIXIT, VISHWA DEEP, MONTGOMERY, RUTH R · 2022 to 2025
$6.5M
Therapeutic strategies for microsatellite expansion diseases using RNA-targeting CRISPR/CasR01NS103172 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MAURICE SCOTT SWANSON, Eugene Wei-Ming Yeo · 2017 to 2026
$5.6M
High-resolution High-speed Photoacoustic and Ultrasound Imaging of SmallVessel Functions in Ischemic StrokeR01NS111039 · NINDS · DUKE UNIVERSITY · PI YAO, JUNJIE · 2019 to 2023
$2.7M
Shear stress Regulation of Endothelial Glycolysis via METTL3-mediated RNA m6A ModificationR01HL170107 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHU CHIEN, John YJ Shyy · 2024 to 2026
$2.5M
Sugar Probed SRS Volumetric imaging of Metabolic ActivitiesR01GM149976 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Lingyan Shi · 2023 to 2026
$1.6M
Novel Optical Imaging Approach to Study Neurovascular Coupling SystemR21NS125395 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHI, LINGYAN · 2022 to 2022
$435k
NCI NIH HHS U54 CA274502NHGRI NIH HHS R01 HG004659NHLBI NIH HHS R01 HL170107NHLBI NIH HHS U54 HL165443NIAID NIH HHS U01 AI167892NIA NIH HHS U54 AG076043NIA NIH HHS U54 AG079759NIDDK NIH HHS U54 DK134301NIGMS NIH HHS R01 GM149976NINDS NIH HHS R01 NS103172NINDS NIH HHS R01 NS111039NINDS NIH HHS R21 NS125395
6 · The paper itself

Abstract

Cells dynamically rewire their metabolic pathways in response to physiological and pathological cues. Such plasticity is particularly critical in neurons, stem cells, cancer cells, and immune cells, where biosynthetic demands can shift rapidly. However, current metabolic imaging techniques using isotope labeling typically track only one metabolite at a time, limiting their ability to capture the rapid dynamics of complex metabolic networks including coordinated precursor utilization, crosstalk, and turnover. Here, we present Subcellular Multiplexed Metabolic Isotope Tracing Stimulated Raman Scattering microscopy (SuMMIT-SRS), a platform that enables simultaneous visualization of multiple metabolic dynamics at subcellular resolution. By exploiting the distinct vibrational signatures of carbon-deuterium bonds derived from multiple deuterated amino acids, lipids, and monosaccharide tracers, SuMMIT-SRS maps co-regulated DNA, RNA, protein, and lipid synthesis at the same time and resolves various individual amino acid-mediated metabolic pathways within intact cells and tissues. We demonstrate SuMMIT's broad utility across

Indexed as

lipidMetabolic rewiringmetabolismmultiplexoptical imagingproteinSRS

Identifiers

PMID41279865
PMCPMC12632847

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.