Evidence map›Paper›PMID 41279683›Full record

ArticlebioRxiv : the preprint server for biology2025

The Age-Dependent Resident Myonuclear Multi-Omic Response to a Skeletal Muscle Hypertrophic Stimulus.

Pieter J Koopmans, Ronald G Jones, Ana Regina Cabrera, Francielly Morena, Nicholas P Greene, John J McCarthy, Ahmed Ismaeel, Yuan Wen, Kevin A Murach

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Pieter J KoopmansCell and Molecular Biology Graduate Program, University of Arkansas, Fayetteville, AR, USA.ORCID 0000-0002-4797-5265
Ronald G JonesMolecular Muscle Mass Regulation (M3R) Laboratory, Department Health, Human Performance, & Recreation, University of Arkansas, Fayetteville, AR, USA.
Ana Regina CabreraMolecular Muscle Mass Regulation (M3R) Laboratory, Department Health, Human Performance, & Recreation, University of Arkansas, Fayetteville, AR, USA.ORCID 0000-0002-9811-3585
Francielly MorenaCachexia Research Laboratory, Department Health, Human Performance, & Recreation, University of Arkansas, Fayetteville, AR, USA.ORCID 0000-0001-5019-2125
Nicholas P GreeneCachexia Research Laboratory, Department Health, Human Performance, & Recreation, University of Arkansas, Fayetteville, AR, USA.ORCID 0000-0001-9621-2005
John J McCarthyDepartment of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.
Ahmed IsmaeelDepartment of Anatomy, Physiology, and Pharmacology, College of Medicine, Auburn University, Auburn, AL, USA.ORCID 0000-0003-4025-3945
Yuan WenDepartment of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.
Kevin A MurachCell and Molecular Biology Graduate Program, University of Arkansas, Fayetteville, AR, USA.

Funding

Understanding Hesitant AdoptersP20GM103429 · NIGMS · UNIV OF ARKANSAS FOR MED SCIS · PI Stephanie Diane Hayes · 2012 to 2026
$60.9M
Unraveling Gene-Environment Interactions Shaping Metabolism: A Multi-Omics Analysis in DrosophilaP20GM139768 · NIGMS · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI Joanna Fiddler · 2021 to 2026
$17.0M
Understanding Cross-National Health Differences at Older Ages and Their CausesR01AG063944 · NIA · NATIONAL BUREAU OF ECONOMIC RESEARCH · PI BOERSCH-SUPAN, AXEL H, CRIMMINS, EILEEN M · 2019 to 2024
$3.2M
Mediators of Muscle Rejuvenation with AgingR01AG080047 · NIA · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI Kevin Murach · 2023 to 2026
$2.3M
Mediators of Muscle Rejuvenation with AgingK02AG088465 · NIA · UNIVERSITY OF MISSOURI-COLUMBIA · PI Kevin Murach · 2025 to 2026
$285k
NIA NIH HHS K02 AG088465NIA NIH HHS R01 AG063944NIA NIH HHS R01 AG080047NIGMS NIH HHS P20 GM103429NIGMS NIH HHS P20 GM139768
6 · The paper itself

Abstract

A detailed analysis of how muscle fiber nuclei (myonuclei) respond to a hypertrophic stimulus would provide a critical step toward understanding compromised skeletal muscle plasticity with age. We used recombination-independent doxycycline-inducible myonucleus-specific fluorescent labelling, tissue RNA-sequencing, myonuclear DNA methylation analysis, multi-omic integration, and single myonucleus RNA-sequencing to define the molecular characteristics of adult (6-8 month) and aged (24 month) murine skeletal muscle after acute mechanical overload (MOV). In adult and aged MOV muscles, we found that: 1) similarities in the transcriptional response to loading - specifically in metabolism genes - were partly explained by a post-transcriptional microRNA-mediated mechanism, which we corroborated using an inducible muscle fiber-specific

Identifiers

PMID41279683
PMCPMC12636604

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.