Evidence map›Paper›PMID 41279651›Full record

ArticlebioRxiv : the preprint server for biology2025

Vulnerability in the breadth evolution of an influenza broadly neutralizing antibody.

Katrine E Dailey, Yiquan Wang, Qi Wen Teo, Chaoyang Wang, Ruipeng Lei, Meixuan Tong, Lucia A Rodriguez, Letianchu Wang, Nicholas C Wu

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Katrine E DaileyDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-0662-0011
Yiquan WangDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-1954-9808
Qi Wen TeoDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Chaoyang WangDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Ruipeng LeiDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Meixuan TongDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Lucia A RodriguezDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Letianchu WangDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Nicholas C WuDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-9078-6697

Funding

Sequence-function relationship of influenza broadly neutralizing antibodiesR01AI167910 · NIAID · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Nicholas C. Wu · 2022 to 2026
$2.5M
High-throughput identification of antibody features for sequence-based epitope predictionDP2AT011966 · NCCIH · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI WU, NICHOLAS C. · 2021 to 2024
$2.4M
NCCIH NIH HHS DP2 AT011966NIAID NIH HHS R01 AI167910
6 · The paper itself

Abstract

The highly conserved influenza hemagglutinin (HA) stem domain is a major target for broadly neutralizing antibodies (bnAbs). However, despite being discovered more than a decade ago, the IGHV1-69-encoded CR9114 remains the only HA stem bnAb that cross-reacts with both influenza A and B viruses. To investigate the constraints on the breadth evolution of CR9114, this study performs four deep mutational scanning experiments to compare the binding affinity landscapes of the germline and somatic CR9114 against H1 HA, H3 HA, and influenza B HA. Many mutations that minimally affect or even improve the H1 HA binding are detrimental for binding to H3 HA and BHA. We further reveal the prevalence of epistasis in IGHV1-69 HA stem bnAbs. Overall, our findings provide a mechanistic explanation for the scarcity of HA stem bnAbs with cross-reactivity against both influenza A and B viruses, and have important implications for developing broadly protective influenza vaccines.

Identifiers

PMID41279651
PMCPMC12637573

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.