Evidence map›Paper›PMID 41279567›Full record

ArticlebioRxiv : the preprint server for biology2025

Modified self-amplifying RNA mediates robust and prolonged gene expression in the mammalian brain.

Jennifer Freire, Joshua E McGee, Dana Shaw, Yuxin Zhou, Yangyang Wang, Colin Porter, Lauren Dang, Erynne San Antonio, Ziqing Yu, Kexin Li and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jennifer FreireDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0002-6811-7438
Joshua E McGeeDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0003-3327-6209
Dana ShawDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0003-1828-9191
Yuxin ZhouDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0003-3665-0999
Yangyang WangDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.
Colin PorterDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0009-0006-1072-3378
Lauren DangDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0009-0003-2865-1155
Erynne San AntonioDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0003-4984-9534
Ziqing YuDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0009-0006-2743-445X
Kexin LiDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0009-0000-8965-1560
Wilson W WongDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0001-8394-889X
Mark W GrinstaffDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0002-5453-3668
Xue HanDepartment of Biomedical Engineering, Boston University, Boston, MA, USA.ORCID 0000-0003-3896-4609

Funding

TRAINING PROGRAM IN QUANTITATIVE BIOLOGY AND PHYSIOLOGYT32GM008764 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI WHITE, JOHN A. · 2001 to 2021
$5.9M
Translational Research in BiomaterialsT32EB006359 · NIBIB · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI MARK W. GRINSTAFF, Michelle H Teplensky · 2009 to 2026
$4.2M
Voltage Imaging Analysis of Striatal Network Dynamics Related to Movement, Parkinson's Disease and Deep Brain StimulationR01NS115797 · NINDS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI HAN, XUE · 2020 to 2024
$2.3M
Optical voltage imaging analysis of the cellular and network mechanisms of deep brain stimulationRF1NS129520 · NINDS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI HAN, XUE · 2022 to 2022
$2.0M
Optical Voltage Imaging Analysis of the Cellular and Network Mechanisms of Deep Brain StimulationR01NS129520 · NINDS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI Xue Han · 2025 to 2026
$1.2M
Graduate Training at the Interface of Neuroscience, Optical Engineering and Data ScienceT32NS136080 · NINDS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI David A Boas, Jerry L Chen · 2024 to 2026
$661k
Advanced laser scanning confocal microscope for multiple usersS10OD024993 · OD · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI MERTZ, JEROME · 2018 to 2018
$448k
Mechanisms of Noninvasive Ultrasonic Neuromodulation of Parkinsonian Neural CircuitsF31NS143166 · NINDS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI SAN ANTONIO, ERYNNE · 2025 to 2025
$42k
NIBIB NIH HHS T32 EB006359NIGMS NIH HHS T32 GM008764NIH HHS S10 OD024993NINDS NIH HHS F31 NS143166NINDS NIH HHS R01 NS115797NINDS NIH HHS R01 NS129520NINDS NIH HHS RF1 NS129520NINDS NIH HHS T32 NS136080
6 · The paper itself

Abstract

In self-amplifying RNA (saRNA), substitution of cytidine with 5-hydroxymethylcytidine (hm5C) reduces innate immune responses and prolongs protein expression. When formulated as a vaccine and administered intramuscularly, lipid nanoparticles (LNPs) loaded with modified saRNA (saRNA-LNPs) afford robust and long-term protein expression. Here we report the protein expression and cell type tropism of modified saRNA-LNPs, encoding fluorescent proteins, when injected in the mammalian brain. saRNA encapsulated in an LNP formulation comprising ALC-0315 (present in Comirnaty

Indexed as

astrocytescentral nervous systemgene transferImmune evasionmodified saRNAneurons

Identifiers

PMID41279567
PMCPMC12637552

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.