ArticlebioRxiv : the preprint server for biology2025
Toward simple, rapid, and deep plant proteome analysis with an in-cell proteomics strategy.
Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Although modern mass spectrometry (MS) based proteomics has been broadly employed by the plant science community, proteomics analysis of plant tissues remains highly challenging to general plant biology laboratories, mainly due to the lack of effective and easily adaptable strategies that can process plant samples for MS analysis rapidly and reliably. Existing methods often involves lengthy mechanical disruption and protein extraction, which not only require large starting materials but also can cause significant variations, compromising quantitation accuracy. Here, we present an "in-cell proteomics" strategy and benchmark its performance against conventional processing methods using several model plants, including
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