Evidence map›Paper›PMID 41279391›Full record

ArticlebioRxiv : the preprint server for biology2025

Visualizing PINK1 Activity Dynamics in Single Cells with a Phase Separation-Based Kinase Activity Reporter.

Katie G Vineall, Alexia Andrikopoulos, Michael J Sun, Anna Yan, Ethan R Hartanto, Danielle L Schmitt

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Katie G VineallDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA USA.ORCID 0009-0007-1113-6594
Alexia AndrikopoulosDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA USA.ORCID 0009-0003-9168-7820
Michael J SunDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA USA.
Anna YanDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA USA.
Ethan R HartantoDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA USA.
Danielle L SchmittDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA USA.ORCID 0000-0002-7343-2125

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Imaging Spatiotemporal Regulation of Acetyl-CoADP2GM154012 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Danielle Lynanne Schmitt · 2023 to 2026
$2.2M
NIGMS NIH HHS DP2 GM154012NIGMS NIH HHS T32 GM007185
6 · The paper itself

Abstract

Phosphatase and tensin homologue-induced kinase 1 (PINK1) is a serine/threonine kinase that plays roles in mitophagy, cell death, and regulation of cellular bioenergetics. Current approaches for studying PINK1 function depend on bulk techniques that can only provide snapshots of activity and could miss the dynamics and cell-to-cell heterogeneity of PINK1 activity. Therefore, we sought to develop a novel PINK1 kinase activity reporter to characterize PINK1 activity. Taking advantage of the separation of phases-based activity reporter of kinase (SPARK) design, we developed a phase separation-based PINK1 biosensor (PINK1-SPARK). With PINK1-SPARK, we observe real-time PINK1 activity in single cells treated with mitochondria depolarizing agents or pharmacological activators. We then developed a Halo Tag-based PINK1-SPARK for multiplexed imaging of PINK1 activity with live-cell markers of mitochondrial damage. Thus, PINK1-SPARK is a new tool that enables temporal measurement of PINK1 activity in single live cells, allowing for further elucidation of the role of PINK1 in mitophagy and cell function.

Indexed as

biosensorfluorescencekinase activity reportermitophagyPINK1

Identifiers

PMID41279391
PMCPMC12632607

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.