Evidence map›Paper›PMID 41279267›Full record

ArticlebioRxiv : the preprint server for biology2025

"A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in

Jordan Scott Summers, Julie Ming Liang, Nolan Warren Kennedy, Danielle Tullman-Ercek

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jordan Scott SummersDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.ORCID 0000-0002-4876-4486
Julie Ming LiangOpera Bioscience, Evanston, Illinois, USA.ORCID 0000-0002-3292-2910
Nolan Warren KennedyDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.ORCID 0000-0002-2944-5439
Danielle Tullman-ErcekDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.ORCID 0000-0001-6734-480X

Funding

Developing Capacity to Evaluate Training Programs via Development of Human, Institutional and Social CapitalT32GM008449 · NIGMS · NORTHWESTERN UNIVERSITY · PI LEONARD, JOSHUA NATHANIEL · 1993 to 2023
$7.8M
NIGMS NIH HHS T32 GM008449
6 · The paper itself

Abstract

Recombinant protein production in bacteria is limited by costly cell lysis and multi-step purification. To bypass these limitations, leveraging native bacterial secretion systems to secrete proteins offers a promising alternative. The type 3 secretion system (T3SS) of

Indexed as

DFHBI:(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-oneIPTG: isopropyl β-d-1-thiogalactopyranosideLB-ES: Luria-Bertani broth (Lennox), enhanced secretionLB-L: Luria-Bertani Broth-LennoxLB-M: Luria-Bertani Broth-MillermFAP2a: mini-Fluorescence Activating ProteinPOI: protein of interestSPI-1: Salmonella Pathogenicity Island-1SR-SIM: Super Resolution-Structured Illumination MicroscopyT3SS: Type Three Secretion System

Identifiers

PMID41279267
PMCPMC12633367

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.