Evidence map›Paper›PMID 41278644›Full record

ArticlebioRxiv : the preprint server for biology2025

Age- and Sex- Driven Transcriptional and Metabolic Diversity in Myeloid-Derived Suppressor Cells After Mouse Sepsis.

Christine Rodhouse, Dayuan Wang, Hongru Tang, Valerie E Polcz, Miguel Hernández-Ríos, Xuanxuan Yu, Leandro Balzano-Nogueira, Jaimar C Rincon, Angel Charles, Seiichiro Fujishima and 21 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Christine RodhouseSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Dayuan WangSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Hongru TangSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Valerie E PolczSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Miguel Hernández-RíosSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Xuanxuan YuSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Leandro Balzano-NogueiraSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Jaimar C RinconSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Angel CharlesSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Seiichiro FujishimaDepartment of Surgery, National Defense Medical College, Namiki, Tokorozawa, Japan.
Quan VoDepartment of Neuroscience, University of Florida College of Medicine, Gainesville, Florida, USA.
Marvin L DirainSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Ricardo UngaroSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Dina C NacionalesSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Leilani Zeumer-SpataroSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Larissa Langhi PrataCenter for Advanced Gerotherapeutics, Division of Endocrinology, Diabetes & Metabolism, Cedars-Sinai Medical Center; Los Angeles, CA, USA.
Shawn D LarsonSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Gemma CasadesusDepartment of Pharmacy and Therapeutics, University of Florida College of Medicine, Gainesville, FL, USA.
Feifei XiaoSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.ORCID 0000-0002-1597-4719
Shannon M WalletDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, FL, USA.
Maigan A BruskoSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Tyler J LoftusSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Letitia BibleSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Alicia M MohrSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Paramita ChakrabartyDepartment of Neuroscience, University of Florida College of Medicine, Gainesville, Florida, USA.
Michael P KladdeSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.ORCID 0000-0001-7951-9291
Clayton E MathewsSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Lyle L MoldawerSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Robert MaileSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.ORCID 0000-0002-9126-4188
Guoshuai CaiSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.
Philip A EfronSepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, FL, USA.ORCID 0000-0002-3931-650X

Funding

Molecular Biology in Burns and TraumaT32GM008721 · NIGMS · UNIVERSITY OF FLORIDA · PI Philip A Efron · 1999 to 2026
$5.0M
Sepsis and the Systemic Cytokine Storm in Aging and Alzheimer Disease ModelsRF1NS128626 · NINDS · UNIVERSITY OF FLORIDA · PI CHAKRABARTY, PARAMITA, EFRON, PHILIP A · 2022 to 2022
$2.2M
Interdisciplinary Training for Vascular Surgeon ScientistsT32HL160491 · NHLBI · UNIVERSITY OF FLORIDA · PI Scott A Berceli, Gilbert Rivers Upchurch · 2022 to 2026
$1.6M
Evaluation of Chorionic Gonadotropin as a Treatment for Sepsis-induced Neuroinflammation and Cognitive Dysfunction in the Aged BrainR21AG087039 · NIA · UNIVERSITY OF FLORIDA · PI CASADESUS, GEMMA · 2024 to 2024
$419k
Senescent Cells Effects in Response to PathogensK01AG090692 · NIA · CEDARS-SINAI MEDICAL CENTER · PI Larissa Gutman Paranhos Langhi Prata · 2025 to 2026
$325k
NHLBI NIH HHS T32 HL160491NIA NIH HHS K01 AG090692NIA NIH HHS R21 AG087039NIGMS NIH HHS T32 GM008721NINDS NIH HHS RF1 NS128626
6 · The paper itself

Abstract

Sepsis induces profound immune dysregulation, often resulting in chronic critical illness characterized by persistent immunosuppression and poor outcomes. Myeloid-derived suppressor cells (MDSCs) are central mediators of this immunosuppressive phenotype, yet the influence of age and sex on their transcriptional and metabolic states remain poorly understood. Here, we employed single-cell RNA sequencing of splenic leukocytes from young (3-4 months) and older (18-24 months) adult male and female mice subjected to a clinically relevant murine sepsis model to define age- and sex-specific MDSC phenotypes. We identified significant differences regarding age and sex in MDSC expansion, transcriptome, canonical pathway activation, RNA velocity, mitochondrial metabolism, and predicted cell-cell communication after sepsis. Using drug2cell analysis of total leukocytes we also identified cohort-specific drug target profiles. These findings underscore the importance of age and sex in shaping sepsis-induced MDSC biology and suggest that personalized immunomodulatory strategies targeting MDSCs could improve sepsis outcomes.

Indexed as

myeloid-derived suppressor cellsprecision medicinesepsis

Identifiers

PMID41278644
PMCPMC12632318

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.