Evidence map›Paper›PMID 41278138›Full record

ArticleAmerican journal of stem cells2025

The role of FN1 gene interference in neural differentiation of human bone marrow mesenchymal stem cells.

Buhe Buren, Chaoqian Han, Chenyuan Yang, Feng Li, Dongsheng Fan, Xiaolong Wang, Xiaosai Hou, Xiaoxu Liu, Shangfei Jing

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of stem cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Buhe BurenDepartment of Hand and Foot Microsurgery Area B, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Chaoqian HanDepartment of Hand and Foot Microsurgery Area B, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Chenyuan YangDepartment of Radiology, The Inner Mongolia Hospital of Peking University Cancer Hospital Hohhot, Inner Mongolia Autonomous Region, China.
Feng LiDepartment of Medical Insurance, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Dongsheng FanDepartment of Hand and Foot Microsurgery Area B, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Xiaolong WangDepartment of Hand and Foot Microsurgery Area B, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Xiaosai HouDepartment of Medical Affairs, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Xiaoxu LiuDepartment of Hand and Foot Microsurgery Area B, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.
Shangfei JingDepartment of Hand and Foot Microsurgery Area B, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, Inner Mongolia Autonomous Region, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveFibronectin 1 (FN1) encodes fibronectin, a protein essential for cell adhesion, migration, extracellular matrix assembly, and regulation of cell differentiation and proliferation. While FN1 has been implicated in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), its role in neural differentiation remains unclear. This study aimed to investigate the effect of FN1 gene interference on neural differentiation of human BMSCs and explore the underlying molecular mechanisms.

methodThree small interfering RNAs (ssi-417, si-4467 and si-5468) targeting FN1 were designed and transfected into BMSCs undergoing neural differentiation. Morphological changes were observed, and FN1 expression was assessed at both mRNA and protein levels. Alkaline phosphatase (ALP) staining was performed, and the expression of neural differentiation-related markers (MAP2, Tuj1, NSE and DCX) was quantified. Transcriptome sequencing was used to identify differentially expressed genes (DEGs), alternative splicing (AS) events and key pathways. Protein-protein interaction (PPI) network analysis was conducted to identify hub genes.

resultCells in the FN1 interference group retained a spindle-shaped mesenchymal morphology. FN1 expression at both mRNA and protein levels was significantly reduced in all three siRNA groups compared with the model group (

conclusionFN1 gene interference inhibits neural differentiation of BMSCs and alters key signaling pathways and splicing patterns, suggesting that FN1 plays a critical role in regulating stem cell fate. These findings provide new insights into the molecular mechanisms underlying neural differentiation of BMSCs.

Indexed as

Bone marrow stem cellsFN1 geneneural differentiationsi-RNA

Identifiers

PMID41278138
PMCPMC12629971

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.