Evidence map›Paper›PMID 41277840›Full record

ArticleJournal of virology2025

The glycosylation variant at residue 381 of the spike protein contributes to virulence shifts in porcine epidemic diarrhea virus during both natural field transmission and laboratory cell passaging with poor cross-protection.

Zhiwei Li, Zhiqian Ma, Yongqi Li, Xiaojing Zhao, Yonghui Zheng, Yang Li, Yingtong Feng, Xuyang Guo, Zifang Zheng, Lele Xu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Zhiwei Li *State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Zhiqian Ma *State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.ORCID 0009-0006-3353-7519
Yongqi Li *State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Xiaojing ZhaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Yonghui ZhengState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Yang LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Yingtong FengState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Xuyang GuoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Zifang ZhengState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Lele XuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Jianwu ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Haixue ZhengState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.ORCID 0000-0001-6850-1379
Shuqi XiaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.ORCID 0000-0002-6468-2608

Funding

Agriculture Research System of China CARS-35China Postdoctoral Science Foundation 2024M753579Joint Research Foundation of Gansu Province 23JRRA1476Joint Research Foundation of Gansu Province 24JRRA804Major Scientific and Technological Special Project of Gansu Province 22ZD6NA001National Natural Science Foundation of China 32402888
6 · The paper itself

Abstract

The virulence and immunogenicity of porcine epidemic diarrhea virus (PEDV) vary during field circulation and cell culture passage (such as when the GI CV777 strain is attenuated through serial passaging). This study revealed that the glycosylation site mutation at position 381 (N381K) of the S protein is associated with these phenomena. Compared with piglets inoculated with P13 virus, piglets inoculated with P100 (N381K) of virulent GX223 exhibited delayed diarrhea, viral shedding, and mortality. Using the virulent rCH/SX/2016-S IMPORTANCE: Porcine epidemic diarrhea virus (PEDV) continues to cause substantial economic losses in the global swine industry, with emerging strains challenging existing vaccine strategies. This study identifies the N381K glycosylation site mutation in the S protein of PEDV as a factor involved in variations in virulence during natural transmission and laboratory adaptation. Crucially, the mutant induces suboptimal neutralizing immunity against the prevalent strain, revealing a mechanism by which classical-strain vaccines may provide limited protection against currently circulating strains. Our findings reveal how a single glycan modification modulates both pathogenicity and immunogenicity, providing critical insights for the development of effective vaccines against circulating PEDV variants.

Indexed as

Coronavirus InfectionsCross ProtectionPorcine epidemic diarrhea virusSpike Glycoprotein, CoronavirusSwine DiseasesAnimalsAntibodies, NeutralizingAntibodies, ViralDiarrheaGlycosylationMutationSerial PassageSwineVirulenceVirus SheddingAntibodies, NeutralizingAntibodies, ViralSpike Glycoprotein, Coronaviruscross-protectionglycosylation mutationporcine epidemic diarrhea virusspike proteinviral pathogenesis

Identifiers

PMID41277840
PMCPMC12724335

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.