ArticleAnalytical chemistry2025
Comprehensive Quantitative Profiling of Less Polar Lipids in Human Plasma Using Validated Reversed-Phase UHPSFC/MS/MS.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The quantitative analysis of lipids is challenging due to their structural diversity and the coexistence of numerous isomers in biological samples. Here, we present a robust and validated method based on reversed-phase ultrahigh-performance supercritical fluid chromatography-tandem mass spectrometry (RP-UHPSFC/MS/MS) for the high-throughput profiling of less polar lipids in human plasma. The sample preparation workflow involves an initial Folch-based extraction, followed by chemical derivatization with benzoyl chloride, and the final liquid-liquid extraction into hexane to isolate less polar acylated analytes. This strategy enables the sensitive profiling of six major lipid classes (TG, DG, MG, SE, ST, and FA) in positive ion mode in less than 18 min. The use of two C18 columns in series provides high chromatographic resolution, sufficient to separate isomeric species, including
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