ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Temporal Transcriptional Regulation of Human Neuronal Differentiation via Forward Programming.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Temporal Transcriptional Regulation of Human Neuronal Differentiation via Forward Programming.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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Authors and funding
14 authors.
Funding
Abstract
Human pluripotent stem cells (hPSCs) serve as a powerful model for studying human neuronal differentiation, yet the temporal control of this process remains poorly understood. This study compares two differentiation systems with distinct timing of differentiation: transcription factor (TF)-induced forward programming and stepwise cellular differentiation by dual-SMAD (DS) inhibition. The analyses reveal that divergent cellular trajectories drive distinct neurogenesis timing. Multi-omic analysis identifies crucial gene regulatory networks (GRNs) that govern cell fate determination and timing control. Perturbation of these GRNs modulates the timing of neurogenesis and neuronal maturation. Specifically, OLIG family TFs, enriched in the TF-induced system, promoted cell cycle exit via NOTCH signaling regulation; their ablation delays neurogenesis in this system. Additionally, NEUROD2 overexpression after neurogenesis accelerated in vitro neuronal maturation in both TF- and DS-induced differentiating cells by enhanced activation of maturation gene modules. These findings elucidate transcriptional mechanisms governing differentiation timing and provide a framework for rationally designing timing-controlled in vitro differentiation strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.